University of Hertfordshire

By the same authors

  • R Louwen
  • D Horst-Kreft
  • A G de Boer
  • L van der Graaf
  • G de Knegt
  • M Hamersma
  • A P Heikema
  • A R Timms
  • B C Jacobs
  • J A Wagenaar
  • H P Endtz
  • J van der Oost
  • J M Wells
  • E E S Nieuwenhuis
  • A H M van Vliet
  • P T J Willemsen
  • P van Baarlen
  • A van Belkum
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Original languageEnglish
Number of pages20
Pages (from-to)207-26
JournalEuropean journal of clinical microbiology & infectious diseases
Journal publication date1 Feb 2013
Volume32
Issue2
Early online date4 Sep 2012
DOIs
StatePublished - 1 Feb 2013

Abstract

Guillain-Barré syndrome (GBS) is a post-infectious disease in which the human peripheral nervous system is affected after infection by specific pathogenic bacteria, including Campylobacter jejuni. GBS is suggested to be provoked by molecular mimicry between sialylated lipooligosaccharide (LOS) structures on the cell envelope of these bacteria and ganglioside epitopes on the human peripheral nerves, resulting in autoimmune-driven nerve destruction. Earlier, the C. jejuni sialyltransferase (Cst-II) was found to be linked to GBS and demonstrated to be involved in the biosynthesis of the ganglioside-like LOS structures. Apart from a role in pathogenicity, we report here that Cst-II-generated ganglioside-like LOS structures confer efficient bacteriophage resistance in C. jejuni. By bioinformatic analysis, it is revealed that the presence of sialyltransferases in C. jejuni and other potential GBS-related pathogens correlated significantly with the apparent degeneration of an alternative anti-virus system: type II Clusters of Regularly Interspaced Short Palindromic Repeat and associated genes (CRISPR-Cas). Molecular analysis of the C. jejuni CRISPR-Cas system confirmed the bioinformatic investigation. CRISPR degeneration and mutations in the cas genes cas2, cas1 and csn1 were found to correlate with Cst-II sialyltransferase presence (p < 0.0001). Remarkably, type II CRISPR-Cas systems are mainly found in mammalian pathogens. To study the potential involvement of this system in pathogenicity, we inactivated the type II CRISPR-Cas marker gene csn1, which effectively reduced virulence in primarily cst-II-positive C. jejuni isolates. Our findings indicate a novel link between viral defence, virulence and GBS in a pathogenic bacterium.

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