University of Hertfordshire

From the same journal

By the same authors

  • A. Davenport
  • M.J. Ashby
  • P. Easton
  • S. Ella
  • J. Bedford
  • Claire Dickerson
  • D.J. Nunez
  • S.J. Capper
  • M.J. Brown
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Original languageEnglish
Pages (from-to)261-264
JournalClinical Science
Journal publication date1990
Publication statusPublished - 1990


1. A radioimmunoassay has been developed for measuring endothelin-like immunoreactivity in human plasma using an antibody raised against endothelin-1 which also cross-reacts with big endothelin-1 and endothelin-2 but not endothelin-3. 2. The sensitivity of the assay was 1 fmol/tube with inter- and intra-assay coefficients of variation of 13% and 9%, respectively. Cross-reactivity with endothelin-3 and non-endothelin peptides was less than 1%. 3. Endothelin-like immunoreactivity was present in the plasma of hypertensive patients (n = 25) at a concentration of 5.7 +/- 0.5 pmol/l (mean +/- SEM), which was not significantly different from that of age-matched control subjects (5.1 +/- 0.5 pmol/l). At these levels, endothelin-1 is unlikely to function as a circulating hormone. 4. Within the normotensive group, the concentration of endothelin-like immunoreactivity in plasma was positively correlated with mean arterial blood pressure, but in hypertensive patients it showed a significant negative correlation.


Original article can be found at: Copyright The Biochemical Society and the Medical Research Society [Full text of this article is not available in the UHRA]

ID: 107934