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A sensitive radioimmunoassay measuring endothelin-like immunoreactivity in human plasma : comparison of levels in patients with essential hypertension and normotensive control subjects. / Davenport, A.; Ashby, M.J.; Easton, P.; Ella, S.; Bedford, J.; Dickerson, Claire; Nunez, D.J.; Capper, S.J.; Brown, M.J.

In: Clinical Science, Vol. 78, 1990, p. 261-264.

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Davenport, A. ; Ashby, M.J. ; Easton, P. ; Ella, S. ; Bedford, J. ; Dickerson, Claire ; Nunez, D.J. ; Capper, S.J. ; Brown, M.J. / A sensitive radioimmunoassay measuring endothelin-like immunoreactivity in human plasma : comparison of levels in patients with essential hypertension and normotensive control subjects. In: Clinical Science. 1990 ; Vol. 78. pp. 261-264.

Bibtex

@article{8f464693d7e64408b004d61874b4cf68,
title = "A sensitive radioimmunoassay measuring endothelin-like immunoreactivity in human plasma: comparison of levels in patients with essential hypertension and normotensive control subjects",
abstract = "1. A radioimmunoassay has been developed for measuring endothelin-like immunoreactivity in human plasma using an antibody raised against endothelin-1 which also cross-reacts with big endothelin-1 and endothelin-2 but not endothelin-3. 2. The sensitivity of the assay was 1 fmol/tube with inter- and intra-assay coefficients of variation of 13{\%} and 9{\%}, respectively. Cross-reactivity with endothelin-3 and non-endothelin peptides was less than 1{\%}. 3. Endothelin-like immunoreactivity was present in the plasma of hypertensive patients (n = 25) at a concentration of 5.7 +/- 0.5 pmol/l (mean +/- SEM), which was not significantly different from that of age-matched control subjects (5.1 +/- 0.5 pmol/l). At these levels, endothelin-1 is unlikely to function as a circulating hormone. 4. Within the normotensive group, the concentration of endothelin-like immunoreactivity in plasma was positively correlated with mean arterial blood pressure, but in hypertensive patients it showed a significant negative correlation.",
author = "A. Davenport and M.J. Ashby and P. Easton and S. Ella and J. Bedford and Claire Dickerson and D.J. Nunez and S.J. Capper and M.J. Brown",
note = "Original article can be found at: http://www.clinsci.org/cs/078/cs0780261.htm Copyright The Biochemical Society and the Medical Research Society [Full text of this article is not available in the UHRA]",
year = "1990",
language = "English",
volume = "78",
pages = "261--264",
journal = "Clinical Science",
issn = "0143-5221",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - A sensitive radioimmunoassay measuring endothelin-like immunoreactivity in human plasma

T2 - comparison of levels in patients with essential hypertension and normotensive control subjects

AU - Davenport, A.

AU - Ashby, M.J.

AU - Easton, P.

AU - Ella, S.

AU - Bedford, J.

AU - Dickerson, Claire

AU - Nunez, D.J.

AU - Capper, S.J.

AU - Brown, M.J.

N1 - Original article can be found at: http://www.clinsci.org/cs/078/cs0780261.htm Copyright The Biochemical Society and the Medical Research Society [Full text of this article is not available in the UHRA]

PY - 1990

Y1 - 1990

N2 - 1. A radioimmunoassay has been developed for measuring endothelin-like immunoreactivity in human plasma using an antibody raised against endothelin-1 which also cross-reacts with big endothelin-1 and endothelin-2 but not endothelin-3. 2. The sensitivity of the assay was 1 fmol/tube with inter- and intra-assay coefficients of variation of 13% and 9%, respectively. Cross-reactivity with endothelin-3 and non-endothelin peptides was less than 1%. 3. Endothelin-like immunoreactivity was present in the plasma of hypertensive patients (n = 25) at a concentration of 5.7 +/- 0.5 pmol/l (mean +/- SEM), which was not significantly different from that of age-matched control subjects (5.1 +/- 0.5 pmol/l). At these levels, endothelin-1 is unlikely to function as a circulating hormone. 4. Within the normotensive group, the concentration of endothelin-like immunoreactivity in plasma was positively correlated with mean arterial blood pressure, but in hypertensive patients it showed a significant negative correlation.

AB - 1. A radioimmunoassay has been developed for measuring endothelin-like immunoreactivity in human plasma using an antibody raised against endothelin-1 which also cross-reacts with big endothelin-1 and endothelin-2 but not endothelin-3. 2. The sensitivity of the assay was 1 fmol/tube with inter- and intra-assay coefficients of variation of 13% and 9%, respectively. Cross-reactivity with endothelin-3 and non-endothelin peptides was less than 1%. 3. Endothelin-like immunoreactivity was present in the plasma of hypertensive patients (n = 25) at a concentration of 5.7 +/- 0.5 pmol/l (mean +/- SEM), which was not significantly different from that of age-matched control subjects (5.1 +/- 0.5 pmol/l). At these levels, endothelin-1 is unlikely to function as a circulating hormone. 4. Within the normotensive group, the concentration of endothelin-like immunoreactivity in plasma was positively correlated with mean arterial blood pressure, but in hypertensive patients it showed a significant negative correlation.

M3 - Article

VL - 78

SP - 261

EP - 264

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

ER -