University of Hertfordshire

An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Standard

An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles. / Hirst, E ; Kaye, Paul H.; Foot, V ; Clark, J M ; Withers, P B .

OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE. ed. / JC Carrano; A Zukauskas. BELLINGHAM : SPIE, 2004. p. 416-423.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harvard

Hirst, E, Kaye, PH, Foot, V, Clark, JM & Withers, PB 2004, An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles. in JC Carrano & A Zukauskas (eds), OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE. SPIE, BELLINGHAM, pp. 416-423. https://doi.org/10.1117/12.578269

APA

Hirst, E., Kaye, P. H., Foot, V., Clark, J. M., & Withers, P. B. (2004). An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles. In JC. Carrano, & A. Zukauskas (Eds.), OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE (pp. 416-423). BELLINGHAM: SPIE. https://doi.org/10.1117/12.578269

Vancouver

Hirst E, Kaye PH, Foot V, Clark JM, Withers PB. An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles. In Carrano JC, Zukauskas A, editors, OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE. BELLINGHAM: SPIE. 2004. p. 416-423 https://doi.org/10.1117/12.578269

Author

Hirst, E ; Kaye, Paul H. ; Foot, V ; Clark, J M ; Withers, P B . / An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles. OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE. editor / JC Carrano ; A Zukauskas. BELLINGHAM : SPIE, 2004. pp. 416-423

Bibtex

@inproceedings{0093f3323a95409bb88cbf206d1df04a,
title = "An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles.",
abstract = "We describe the construction of a bio-aerosol monitor designed to capture and record intrinsic fluorescence spectra from individual aerosol particles carried in a sample airflow and to simultaneously capture data relating to the spatial distribution of elastically scattered light from each particle. The spectral fluorescence data recorded by this PFAS (Particle Fluorescence and Shape) monitor contains information relating to the particle material content and specifically to possible biological fluorophores. The spatial scattering data from PFAS yields information relating to particle size and shape. The combination of these data can provide a means of aiding the discrimination of bio-aerosols from background or interferent aerosol particles which may have similar fluorescence properties but exhibit shapes and/or sizes not normally associated with biological particles. The radiation used both to excite particle fluorescence and generate the necessary spatially scattered light flux is provided by a novel compact UV fiber laser operating at 266nm wavelength. Particles drawn from the ambient environment traverse the laser beam in single file. Intrinsic particle fluorescence in the range 300-570nm is collected via an ellipsoidal concentrator into a concave grating spectrometer, the spectral data being recorded using a 16-anode linear array photomultiplier detector. Simultaneously, the spatial radiation pattern scattered by the particle over 5degrees-30degrees scattering angle and 360degrees of azimuth is recorded using a custom designed 31-pixel radial hybrid photodiode array. Data from up to similar to5,000 particles per second may be acquired for analysis, usually performed by artificial neural network classification.",
keywords = "bio-aerosol, detection, spectral fluorescence, spatial light scattering, fibre laser, particle shape, particle classification, LIGHT-SCATTERING PROFILES, AIRBORNE PARTICLES, INTRINSIC FLUORESCENCE, BIOLOGICAL PARTICLES, CLASSIFICATION, SYSTEM, LASER",
author = "E Hirst and Kaye, {Paul H.} and V Foot and Clark, {J M} and Withers, {P B}",
note = "Full text of this item is not available in the UHRA",
year = "2004",
doi = "10.1117/12.578269",
language = "English",
isbn = "0-8194-5570-9",
pages = "416--423",
editor = "JC Carrano and A Zukauskas",
booktitle = "OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE",
publisher = "SPIE",

}

RIS

TY - GEN

T1 - An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles.

AU - Hirst, E

AU - Kaye, Paul H.

AU - Foot, V

AU - Clark, J M

AU - Withers, P B

N1 - Full text of this item is not available in the UHRA

PY - 2004

Y1 - 2004

N2 - We describe the construction of a bio-aerosol monitor designed to capture and record intrinsic fluorescence spectra from individual aerosol particles carried in a sample airflow and to simultaneously capture data relating to the spatial distribution of elastically scattered light from each particle. The spectral fluorescence data recorded by this PFAS (Particle Fluorescence and Shape) monitor contains information relating to the particle material content and specifically to possible biological fluorophores. The spatial scattering data from PFAS yields information relating to particle size and shape. The combination of these data can provide a means of aiding the discrimination of bio-aerosols from background or interferent aerosol particles which may have similar fluorescence properties but exhibit shapes and/or sizes not normally associated with biological particles. The radiation used both to excite particle fluorescence and generate the necessary spatially scattered light flux is provided by a novel compact UV fiber laser operating at 266nm wavelength. Particles drawn from the ambient environment traverse the laser beam in single file. Intrinsic particle fluorescence in the range 300-570nm is collected via an ellipsoidal concentrator into a concave grating spectrometer, the spectral data being recorded using a 16-anode linear array photomultiplier detector. Simultaneously, the spatial radiation pattern scattered by the particle over 5degrees-30degrees scattering angle and 360degrees of azimuth is recorded using a custom designed 31-pixel radial hybrid photodiode array. Data from up to similar to5,000 particles per second may be acquired for analysis, usually performed by artificial neural network classification.

AB - We describe the construction of a bio-aerosol monitor designed to capture and record intrinsic fluorescence spectra from individual aerosol particles carried in a sample airflow and to simultaneously capture data relating to the spatial distribution of elastically scattered light from each particle. The spectral fluorescence data recorded by this PFAS (Particle Fluorescence and Shape) monitor contains information relating to the particle material content and specifically to possible biological fluorophores. The spatial scattering data from PFAS yields information relating to particle size and shape. The combination of these data can provide a means of aiding the discrimination of bio-aerosols from background or interferent aerosol particles which may have similar fluorescence properties but exhibit shapes and/or sizes not normally associated with biological particles. The radiation used both to excite particle fluorescence and generate the necessary spatially scattered light flux is provided by a novel compact UV fiber laser operating at 266nm wavelength. Particles drawn from the ambient environment traverse the laser beam in single file. Intrinsic particle fluorescence in the range 300-570nm is collected via an ellipsoidal concentrator into a concave grating spectrometer, the spectral data being recorded using a 16-anode linear array photomultiplier detector. Simultaneously, the spatial radiation pattern scattered by the particle over 5degrees-30degrees scattering angle and 360degrees of azimuth is recorded using a custom designed 31-pixel radial hybrid photodiode array. Data from up to similar to5,000 particles per second may be acquired for analysis, usually performed by artificial neural network classification.

KW - bio-aerosol

KW - detection

KW - spectral fluorescence

KW - spatial light scattering

KW - fibre laser

KW - particle shape

KW - particle classification

KW - LIGHT-SCATTERING PROFILES

KW - AIRBORNE PARTICLES

KW - INTRINSIC FLUORESCENCE

KW - BIOLOGICAL PARTICLES

KW - CLASSIFICATION

KW - SYSTEM

KW - LASER

U2 - 10.1117/12.578269

DO - 10.1117/12.578269

M3 - Conference contribution

SN - 0-8194-5570-9

SP - 416

EP - 423

BT - OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE

A2 - Carrano, JC

A2 - Zukauskas, A

PB - SPIE

CY - BELLINGHAM

ER -