University of Hertfordshire

From the same journal

By the same authors

Documents

View graph of relations
Original languageEnglish
Article number15849
JournalScientific Reports
Journal publication date20 Nov 2017
Volume7
DOIs
Publication statusPublished - 20 Nov 2017

Abstract

Lanosterol 14-α demethy
lase is a key enzyme intermediating the biosynthesis of ergosterol in fungi,
and the target of azole fungicides. Studies have suggested that Leptosphaeria maculans and L.
biglobosa, the causal agents of phoma stem canker on oilseed rape, differ in their sensitivity to some
azoles, which could be driving pathogen frequency change in crops. Here we used CYP51 protein
modelling and heterologous expression to determine whether there are interspecific differences at the
target-site level. Moreover, we provide an example of intrinsic sensitivity differences exhibited by both
Leptosphaeria spp. in vitro and in planta. Comparison of homologous protein models identified highly
conserved residues, particularly at the azole binding site, and heterologous expression of LmCYP51B
and LbCYP51B, with fungicide sensitivity testing of the transformants, suggests that both proteins are
similarly sensitive to azole fungicides flusilazole, prothioconazole-desthio and tebuconazole. Fungicide
sensitivity testing on isolates shows that they sometimes have a minor difference in sensitivity in vitro
and in planta. These results suggest that azole fungicides remain a useful component of integrated
phoma stem canker control in the UK due to their effectiveness on both Leptosphaeria spp. Other
factors, such as varietal resistance or climate, may be driving observed frequency changes between
species.

Notes

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017

ID: 12728300