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Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi. / Casassola, Alice; Brammer, Sandra P.; Chaves, Márcia S.; Martinelli, José A.; Stefanato, Francesca; Boyd, Lesley.

In: Physiological and Molecular Plant Pathology, Vol. 89, 01.2015, p. 49-54.

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Casassola, Alice ; Brammer, Sandra P. ; Chaves, Márcia S. ; Martinelli, José A. ; Stefanato, Francesca ; Boyd, Lesley. / Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi. In: Physiological and Molecular Plant Pathology. 2015 ; Vol. 89. pp. 49-54.

Bibtex

@article{ef909d13843f432dabe4e2707fe1e780,
title = "Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi",
abstract = "Leaf rust, caused by the foliar pathogen Puccinia triticina is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between P.triticina and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.",
keywords = "APR, AQP1, COMT1, ETI, FREX, G6DPH, GAPDH, Hai, HSP80, LHC, LTP, MIP, NADPH, PAL, PR, PRA2, Pre-haustorial, PTI, Puccinia triticina, QPCR, Quantitative PCR, RBR1, ROS, Triticum aestivum (L.) thell, WCAB, Wheat breeding, ZIP5",
author = "Alice Casassola and Brammer, {Sandra P.} and Chaves, {M{\'a}rcia S.} and Martinelli, {Jos{\'e} A.} and Francesca Stefanato and Lesley Boyd",
year = "2015",
month = "1",
doi = "10.1016/j.pmpp.2014.12.004",
language = "English",
volume = "89",
pages = "49--54",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Changes in gene expression profiles as they relate to the adult plant leaf rust resistance in the wheat cv. Toropi

AU - Casassola, Alice

AU - Brammer, Sandra P.

AU - Chaves, Márcia S.

AU - Martinelli, José A.

AU - Stefanato, Francesca

AU - Boyd, Lesley

PY - 2015/1

Y1 - 2015/1

N2 - Leaf rust, caused by the foliar pathogen Puccinia triticina is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between P.triticina and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.

AB - Leaf rust, caused by the foliar pathogen Puccinia triticina is a major disease of wheat in the southern region of Brazil and invariably impacts on production, being responsible for high yield losses. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust, which uniquely shows a pre-haustorial resistance phenotype. In this study we aimed to understand the interaction between P.triticina and the pre-haustorial APR in Toropi by quantitatively evaluating the temporal transcription profiles of selected genes known to be related to infection and defense in wheat. The expression profiles of 15 selected genes varied over time, grouping into six expression profile groups. The expression profiles indicated the induction of classical defence pathways in response to pathogen development, but also the potential modification of Toropi's cellular status for the benefit of the pathogen. Classical defence genes, including peroxidases, β-1,3-glucanases and an endochitinase were expressed both early (pre-haustorial) and late (post-haustorial) over the 72h infection time course, while induction of transcription of other infection-related genes with a potential role in defence, although variable was maintained through-out. These genes directly or indirectly had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport, and cell cycle regulation. The early induction of transcription of defence-related genes supports the pre-haustorial resistance phenotype in Toropi, providing a valuable source of genes controlling leaf rust resistance for wheat breeding.

KW - APR

KW - AQP1

KW - COMT1

KW - ETI

KW - FREX

KW - G6DPH

KW - GAPDH

KW - Hai

KW - HSP80

KW - LHC

KW - LTP

KW - MIP

KW - NADPH

KW - PAL

KW - PR

KW - PRA2

KW - Pre-haustorial

KW - PTI

KW - Puccinia triticina

KW - QPCR

KW - Quantitative PCR

KW - RBR1

KW - ROS

KW - Triticum aestivum (L.) thell

KW - WCAB

KW - Wheat breeding

KW - ZIP5

U2 - 10.1016/j.pmpp.2014.12.004

DO - 10.1016/j.pmpp.2014.12.004

M3 - Article

AN - SCOPUS:84920913860

VL - 89

SP - 49

EP - 54

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

SN - 0885-5765

ER -