University of Hertfordshire

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Characterization of microvesicles released from cells constitutively and upon stimulation. / Stratton, Dan; Antwi-Baffour, Samuel; Williams, Gareth; Lange, Sigrun; Inal, Jameel.

2012. 2-2 Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Research output: Contribution to conferenceAbstract

Harvard

Stratton, D, Antwi-Baffour, S, Williams, G, Lange, S & Inal, J 2012, 'Characterization of microvesicles released from cells constitutively and upon stimulation' International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden, 18/04/12 - 21/04/12, pp. 2-2.

APA

Stratton, D., Antwi-Baffour, S., Williams, G., Lange, S., & Inal, J. (2012). Characterization of microvesicles released from cells constitutively and upon stimulation. 2-2. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Vancouver

Stratton D, Antwi-Baffour S, Williams G, Lange S, Inal J. Characterization of microvesicles released from cells constitutively and upon stimulation. 2012. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Author

Stratton, Dan ; Antwi-Baffour, Samuel ; Williams, Gareth ; Lange, Sigrun ; Inal, Jameel. / Characterization of microvesicles released from cells constitutively and upon stimulation. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.1 p.

Bibtex

@conference{640b27663495483fa55cffa3bad229b8,
title = "Characterization of microvesicles released from cells constitutively and upon stimulation",
abstract = "Constitutively released microvesicles (cMVs) are released as a part ofnormal cell physiology. However, stimulated microvesicles (sMVs) arereleased as a result of a number of possible stress factors. We foundsMVs to be released in higher numbers than cMVs, typically 10-foldhigher numbers, in the same time frame, and where the stress factorwas a pharmacological agent, the microvesiculation was an attemptto release this chemical stress factor. Using a mass sensingtechnique, the sMVs were released over a 15-minutes period afterstimulation. Using sizing beads on a flow cytometer and bytransmission electron microscopy, the cMVs were typically smaller(less than 300 nm in diameter) than sMVs (300500 nm in diameter).However, cMVs were found to carry more protein. By contrast,phosphatidylserine expression was greater on the larger sMVs, whichalso more effectively inhibited complement-mediated lysis.",
author = "Dan Stratton and Samuel Antwi-Baffour and Gareth Williams and Sigrun Lange and Jameel Inal",
year = "2012",
month = "4",
day = "15",
language = "English",
pages = "2--2",
note = "International Society for Extracellular Vesicles 1st annual meeting : ISEV meeting, 2012, Gothenburg, ISEV, 2012 ; Conference date: 18-04-2012 Through 21-04-2012",
url = "https://www.gu.se/digitalAssets/1367/1367704_scientific-program-gothenburg-april-2012-finalmwii--1-.pdf",

}

RIS

TY - CONF

T1 - Characterization of microvesicles released from cells constitutively and upon stimulation

AU - Stratton, Dan

AU - Antwi-Baffour, Samuel

AU - Williams, Gareth

AU - Lange, Sigrun

AU - Inal, Jameel

PY - 2012/4/15

Y1 - 2012/4/15

N2 - Constitutively released microvesicles (cMVs) are released as a part ofnormal cell physiology. However, stimulated microvesicles (sMVs) arereleased as a result of a number of possible stress factors. We foundsMVs to be released in higher numbers than cMVs, typically 10-foldhigher numbers, in the same time frame, and where the stress factorwas a pharmacological agent, the microvesiculation was an attemptto release this chemical stress factor. Using a mass sensingtechnique, the sMVs were released over a 15-minutes period afterstimulation. Using sizing beads on a flow cytometer and bytransmission electron microscopy, the cMVs were typically smaller(less than 300 nm in diameter) than sMVs (300500 nm in diameter).However, cMVs were found to carry more protein. By contrast,phosphatidylserine expression was greater on the larger sMVs, whichalso more effectively inhibited complement-mediated lysis.

AB - Constitutively released microvesicles (cMVs) are released as a part ofnormal cell physiology. However, stimulated microvesicles (sMVs) arereleased as a result of a number of possible stress factors. We foundsMVs to be released in higher numbers than cMVs, typically 10-foldhigher numbers, in the same time frame, and where the stress factorwas a pharmacological agent, the microvesiculation was an attemptto release this chemical stress factor. Using a mass sensingtechnique, the sMVs were released over a 15-minutes period afterstimulation. Using sizing beads on a flow cytometer and bytransmission electron microscopy, the cMVs were typically smaller(less than 300 nm in diameter) than sMVs (300500 nm in diameter).However, cMVs were found to carry more protein. By contrast,phosphatidylserine expression was greater on the larger sMVs, whichalso more effectively inhibited complement-mediated lysis.

M3 - Abstract

SP - 2

EP - 2

ER -