University of Hertfordshire

Development and Application of Molecular Methods for the Study of Polymyxa betae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

  • Euphemia Mutasa-Gottgens
  • D. M. Chwarszczynska
  • G. Williams
  • E. Ward
  • M. J. Adams
  • M. J. C. Asher
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Original languageEnglish
Title of host publicationProcs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors
EditorsJ.L. Sherwood, C.M. Rush
PublisherAmerican Society of Sugar Beet Technologists
Pages173-176
ISBN (Print)0-926195-02-6
Publication statusPublished - 1996
Event3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors - Dundee, United Kingdom
Duration: 6 Aug 19967 Aug 1996

Conference

Conference3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors
CountryUnited Kingdom
CityDundee
Period6/08/967/08/96

Abstract

A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. This fragment proved to be a good probe for detecting the fungus by Southern/dot blot hybridisation, and a good source of PCR primer sequences for the specific amplification of P.betae DNA. PCR is now used routinely for detecting P.betae in sugar-beet roots and in experiments investigating the epidemiology of the fungus.
Single copy DNA probes have also been isolated and are beginning to provide useful information on variability at the molecular level

ID: 1913387