University of Hertfordshire

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Endotoxin detection in end-stage kidney disease

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Original languageEnglish
Number of pages6
Pages (from-to)73-78
JournalJournal of Clinical Pathology
Journal publication date1 Jan 2015
Early online date6 Nov 2014
Publication statusPublished - 1 Jan 2015


Aims: Endotoxin detection assays are not validated for use in end-stage kidney disease (ESKD). We investigated the accuracy and precision of the kinetic turbidimetric Limulus amoebocyte lysate (LAL) assay to detect endotoxin in plasma from patients with ESKD. Optimisation of endotoxin recovery from plasma using the detergent Tween 80 was also explored. Methods: Plasma samples from 7 patients with ESKD and 7 healthy subjects were spiked with different concentrations of endotoxin. Repeated measurements for endotoxin at each level of spike were performed to assess the accuracy and precision of spike recovery. Endotoxin recovery in plasma samples diluted in Tween 80 and water was compared. Results: Mean endotoxin spike recovery was 111.6% and 125.2% in ESKD and healthy subjects, respectively. There was no statistical difference in spike recovery between ESKD and healthy plasma. Precision of the LAL assay in plasma spiked with low (0.05 EU/mL) and high (0.5 EU/mL) concentration of endotoxin spikes was 24.1% and 8.9%, respectively. The use of Tween 80 as a diluent for plasma significantly improved spike recovery in ESKD plasma (100.1% vs 70.4%, p


Wong J, Jeraj H, Vilar E, et al. 'Endotoxin detection in end-stage kidney disease', Journal of Clinical Pathology 2015;68:73-78, the final published version is available online at doi: Published by the BMJ Publishing Group Limited.

ID: 9608315