University of Hertfordshire

  • Victoria Hutter
  • Natasha Tang
  • Vanessa Zann
  • Anne Cooper
  • David Pritchard
  • Cynthia Bosquillon
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Original languageEnglish
Pages1230-1232
Number of pages1
Publication statusPublished - 2010
EventUK Pharm Sci 2010 - Nottingham, United Kingdom
Duration: 1 Sep 20103 Sep 2010

Conference

ConferenceUK Pharm Sci 2010
CountryUnited Kingdom
CityNottingham
Period1/09/103/09/10

Abstract

The rat airway epithelial cell line RL65 was assessed for its potential as an in-vitro permeability screening tool of inhaled compounds. Cells were grown on Transwell® cell culture supports at an air-liquid interface in serum-free medium. Cell layers exhibited transepithelial electrical resistance (TEER) values >200Ω.cm2 after 14 days in culture when seeded at a density of 1 x 104 cells/cm2. A coefficient of apparent permeability (Papp) ~2.5 x 10-6 cm/s was obtained for the paracellular markers, fluorescein sodium and 14C-mannitol. No vectorial transport was observed for the drug transporter substrates rhodamine 123 and 3H-digoxin.

ID: 824478