University of Hertfordshire

Documents

  • Ioanna Danai Styliari
  • Claudia Conte
  • Amanda Pearce
  • Amanda Huesler
  • Robert Cavanagh
  • Marion Limo
  • Dipak Gordhan
  • Alejandro Nieto-Orellana
  • Jiraphong Suksiriworapong
  • Benoit Couturaud
  • Phil Williams
  • Andrew Hook
  • Morgan R. Alexander
  • Martin Garnett
  • Cameron Alexander
  • Jonathan Burley
  • Vincenzo Taresco
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Original languageEnglish
Article number1800146
JournalMacromolecular Materials and Engineering
Journal publication date1 Aug 2018
Volume303
Issue8
Early online date27 May 2018
DOIs
Publication statusPublished - 1 Aug 2018

Abstract

The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a picoliter inkjet printer is presented. By dispensing polymer solutions in dimethyl sulfoxide (DMSO) from the printer into the wells of a 96‐well plate, containing water as an antisolvent, 50 suspensions are screened for nanoparticle formation rapidly using only nanoliters to microliters. A variety of polymer classes are used and in situ characterization of the submicroliter nanosuspensions shows that the particle size distributions match those of nanoparticles made from bulk suspensions. Dispensing organic polymer solutions into well plates via the printer is thus shown to be a reproducible and fast method for screening nanoparticle formation which uses two to three orders of magnitude less material than conventional techniques. Finally, a pilot study for a high‐throughput pipeline of nanoparticle production, physical property characterization, and cytocompatibility demonstrates the feasibility of the printing approach for screening of nanodrug delivery formulations. Nanoparticles are produced in the well plates, characterized for size and evaluated for effects on metabolic activity of lung cancer cells.

Notes

This is the peer reviewed version of the following article:Ioanna D. Styliari, et al, ‘High‐Throughput Miniaturized Screening of Nanoparticle Formation via Inkjet Printing’, Macromolecular Materials and Engineering, (2018), which has been published in final form at https://doi.org/10.1002/mame.201800146. Under embargo until 27 May 2019. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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