University of Hertfordshire

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Original languageEnglish
Number of pages1
Publication statusPublished - 30 May 2016
EventThe Fifth International Meeting of ISEV, ISEV2016,
Rotterdam, The Netherlands, 4 – 7 May, 2016: ISEV annual meeting, 2016
- Rotterdam, Netherlands
Duration: 4 May 20167 May 2016


ConferenceThe Fifth International Meeting of ISEV, ISEV2016,
Rotterdam, The Netherlands, 4 – 7 May, 2016
Abbreviated titleISEV, 2016
Internet address


Introduction: Microvesicle (MV) release from tumour cells plays an
important role in cancer drug resistance. It is essential that chemotherapeutic
drugs are retained within target cells for increased
effectiveness in inducing apoptosis. Microvesiculation influences drug
retention so minimizing drug efflux is important. Based on MV
biogenesis pathways, a range of potential inhibitors were tested.
Methods: The NanoSight LM10 was used to analyse MV release from
PC3 human prostate cancer cells. Cells were maintained in serumfree
RPMI 1640. Washed cells were seeded in triplicate at 3.8105
cells/well initially treated with 300 mM BzATP for 1 h, and further 1 h
with relevant concentrations of MV-inhibitors. MV count, annexin V
staining and cell viability were assessed. Results: The numbers of
MVs released were compared to control (BzATP alone). Up to 60%
clear inhibition of MV release was shown with all reagents used.
The maximum MV inhibition was with 500 mM ethylene glycol
tetraacetic acid (EGTA) and 10 mM bisindolymaleimide I, resulting
in 48 and 34%, respectively. Cell viability was 80% in all cases
except for panthethine, which only resulted in 25%. Annexin V
staining confirmed the vesicles identified were indeed MVs. Summary/
conclusion: EGTA and bisindolymaleimide I are potent inhibitors of MV
release. Both novel and classic reagents described may be used
individually or in combination. This could be similar to calpeptin
and chloramidine, which enabled prostate cancer cells to sensitize to
docetaxel treatment previously. This study extends the range of
inhibitors that can be utilized to enhance novel combinatory
treatment options.

ID: 13314515