University of Hertfordshire


  • Claudia Sisa
  • Sharad Kholia
  • Jordan Naylor
  • Maria Beatriz Herrera Sanchez
  • Stefania Bruno
  • Maria Chaiara Deregibus
  • Giovanni Camussi
  • Jameel Inal
  • Sigrun Lange
  • Mariya Hristova
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Original languageEnglish
Article number282
Number of pages10
Pages (from-to)282
JournalFrontiers in Physiology
Journal publication date19 Mar 2019
Publication statusPublished - 19 Mar 2019


Background: Neonatal hypoxic-ischemic (HI) insult is a leading cause of disability and death in newborns, with therapeutic hypothermia being the only currently available clinical intervention. Thus there is a great need for adjunct and novel treatments for enhanced or alternative post-HI neuroprotection. Extracellular vesicles (EVs) derived from mesenchymal stromal/stem cells (MSCs) have recently been shown to exhibit regenerative effects in various injury models. Here we present findings showing neuroprotective effects of MSC-derived EVs in the Rice-Vannucci model of severe HI-induced neonatal brain insult.

Methods: Mesenchymal stromal/stem cell-derived EVs were applied intranasally immediately post HI-insult and behavioral outcomes were observed 48 h following MSC-EV treatment, as assessed by negative geotaxis. Brains were thereafter excised and assessed for changes in glial responses, cell death, and neuronal loss as markers of damage at 48 h post HI-insult.

Results: Brains of the MSC-EV treated group showed a significant decrease in microglial activation, cell death, and percentage tissue volume loss in multiple brain regions, compared to the control-treated groups. Furthermore, negative geotaxis test showed improved behavioral outcomes at 48 h following MSC-EV treatment.

Conclusion: Our findings highlight the clinical potential of using MSC-derived EVs following neonatal hypoxia-ischaemia.


© 2019 Sisa, Kholia, Naylor, Herrera Sanchez, Bruno, Deregibus, Camussi, Inal, Lange and Hristova.

ID: 16470868