University of Hertfordshire

Documents

  • Emilie Dumas
  • Célestine M. Atyame
  • Colin A. Malcolm
  • Gilbert Le Goff
  • Sandra Unal
  • Patrick Makoundou
  • Nicole Pasteur
  • Mylène Weill
  • Olivier Duron
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Original languageEnglish
Number of pages10
Pages (from-to)800-809
JournalInsect Molecular Biology
Journal publication date1 Dec 2016
Volume25
Issue6
Early online date3 Sep 2016
DOIs
Publication statusPublished - 1 Dec 2016

Abstract

The Culex pipiens mosquito complex is a group of evolutionarily closely related species including the common house mosquito, Cx. pipiens, and the southern house mosquito, Cx. quinquefasciatus, which both are infected by the cytoplasmically inherited Wolbachia symbiont. A Wolbachia-uninfected population of Cx. pipiens was however described in South Africa and was recently proposed to represent a cryptic species where Wolbachia spread has been prevented by reproductive isolation. In this study, we reconsider the existence of this novel species by undertaking an extensive screening for the presence of Wolbachia-uninfected Cx. pipiens specimens and by characterizing their genetic relatedness with known members of the complex. We first reported on the presence of Wolbachia-uninfected specimens in several Cx. pipiens breeding sites in Europe and North Africa. Using a multi-locus typing scheme, we next confirm that these uninfected specimens unambiguously belong to the Cx. pipiens complex. While, uninfected specimens shared ancestral nuclear DNA polymorphism with infected Cx. pipiens specimens, they also harbor novel mitochondrial haplotypes which are closely related, but different, to those found in all other Cx. pipiens complex members. Overall, these results corroborate the presence of a cryptic species within the Cx. pipiens complex where ancestral levels of mitochondrial diversity have been maintained. We further evidence a geographic distribution far wider than previously suspected, ranging from the North of Europe to the South of Africa.

Notes

This is the peer reviewed version of the following article: Dumas, E., Atyame, C. M., Malcolm, C. A., Le Goff, G., Unal, S., Makoundou, P., Pasteur, N., Weill, M. and Duron, O. (2016), Molecular data reveal a cryptic species within the Culex pipiens mosquito complex. Insect Mol Biol, 25: 800–809,which has been published in final form at doi:10.1111/imb.12264. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. © 2016 The Royal Entomological Society.

ID: 9983580