University of Hertfordshire

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Original languageEnglish
Number of pages6
Pages (from-to)747-752
JournalBiochemical and Biophysical Research Communications
Journal publication date14 Feb 1984
Volume118
Issue3
DOIs
Publication statusPublished - 14 Feb 1984

Abstract

A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme

ID: 1957706