University of Hertfordshire


  • K Charaabi
  • S Boukhris-Bouhachem
  • M Makni
  • Ian Denholm
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Original languageEnglish
Number of pages5
Pages (from-to)1297-1301
JournalPest Management Science
Journal publication date1 Jun 2018
Early online date19 Dec 2017
Publication statusPublished - 1 Jun 2018


BACKGROUND: The R81T mutation conferring target-site resistance to neonicotinoid insecticides in Myzus persicae was first detected in France and has since spread across much of southern Europe. In response to recent claims of control failure with neonicotinoids in Tunisia, we have used a molecular assay to investigate the presence and distribution of this target-site mutation in samples collected from six locations and six crops attacked by M. persicae. RESULTS: The resistance allele containing R81T was present at substantial frequencies (32–55%) in aphids collected between 2014 and 2016 from northern Tunisia but was much rarer further south. It occurred in aphids collected from the aphid's primary host (peach) and four secondary crop hosts (potato, pepper, tomato and melon). Its absence in aphids from tobacco highlights complexities in the systematics of M. persicae that require further investigation. CONCLUSION: This first report of R81T from North Africa reflects a continuing expansion of its range around the Mediterranean Basin, although it remains unrecorded elsewhere in the world. Loss of efficacy of neonicotinoids presents a serious threat to the sustainability of aphid control.


This is the peer reviewed version of the following article: Kamel Charaabi, Sonia Boukhris-bouhachem, Mohamed Makni, and Ian Denholm, ‘Occurrence of target‐site resistance to neonicotinoids in the aphid Myzus persicae in Tunisia, and its status on different host plants’, Pest Management Science, Vol. 74(6): 1297-1301, June 2018, which has been published in final form at Under embargo until 19 December 2018. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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