University of Hertfordshire

  • Sigrun Lange
  • Jameel Inal
  • Selina Wray
  • michael devine
  • Mar Matarin
  • Anthony Nicholas
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Original languageEnglish
Publication statusPublished - 20 Mar 2017
EventLeonard Wolfson Experimental Neurology Centre Annual Symposium - UCL Institute of Child Health, London, U.K., London, United Kingdom
Duration: 20 Mar 201720 Mar 2017
https://www.ucl.ac.uk/lwenc/documents/neuroinflammationsymposium

Conference

ConferenceLeonard Wolfson Experimental Neurology Centre Annual Symposium
CountryUnited Kingdom
CityLondon
Period20/03/1720/03/17
Internet address

Abstract

Background: Progressive neurodegenerative diseases are often referred to as protein misfolding disorders, as common features are calcium dysregulation and accumulated misfolded protein aggregates that lead to fatal neuronal loss. Peptidylarginine deiminases (PADs) are a group of Ca+2 activated enzymes which cause irreversible post-translational conversions of protein bound arginines to citrullines in target proteins, changing their structure and function. Novel roles have been shown for PADs in acute CNS damage, where pharmacological PADinhibition resulted in significant dampening of neuroinflammatory microglial activation and neuroprotective effects in vivo. We have also shown a novel mechanistic role for PADs in the biogenesis of extracellular vesicle (EV) release, via deimination of cytoskeletal proteins and histones, which can be effectively targeted using PAD-inhibitors. Protein deimination and extracellular vesicle release are both Ca2+ mediated mechanisms which are increasingly associated to neurodegenerative diseases. Methods: We are currently using human induced pluripotent stem cell (iPSC) models to elucidate the involvement of PADs and post-translational protein deimination in neurodegenerative disease progression. We are testing neuronal cell lysates, generated from fibroblasts derived from patients carrying mutations for Parkinson’s disease (PD), frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) for expression of PAD isozymes, changes in deiminated target proteins and EV release. Results: We have found that protein deimination is increased in cell lines carrying certain FTD-ALS linked valosin-containing protein mutations (VCPR155C and VCPR191Q) and in the Parkinson Disease α¬ synuclein triplication. Specific targets identified include nuclear histone H3, which is involved in gene regulation and EV release. Conclusion: Human iPSC neurodegenerative disease models can be used to investigate PADmediated mechanisms involved in neurodegenerative pathologies and to test pharmacological PAD-inhibitors for intervention in neurodegenerative disease progression.

ID: 13221093