University of Hertfordshire

From the same journal

By the same authors

  • Dan Stratton
  • Colin Moore
  • Lei Zheng
  • Sigrun Lange
  • Jameel Inal
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Original languageEnglish
Number of pages7
Pages (from-to)511-517
JournalBiochemical and Biophysical Research Communications
Journal publication date8 May 2015
Volume460
Issue3
Early online date19 Mar 2015
DOIs
Publication statusPublished - 8 May 2015

Abstract

MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMs-1 over 11 s, reaching a peak [Ca2+]i of 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMs-1 over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at ∼430 nM.

ID: 13068203