University of Hertfordshire

From the same journal

By the same authors

View graph of relations
Original languageEnglish
Number of pages6
Pages (from-to)470-5
JournalBiochemical and Biophysical Research Communications
Journal publication date11 Jan 2013
Volume430
Issue2
DOIs
Publication statusPublished - 11 Jan 2013

Abstract

Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.

ID: 13069366