University of Hertfordshire

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Original languageEnglish
Number of pages14
Pages (from-to)151-64
JournalMethods in Molecular Biology
Journal publication date2008
Publication statusPublished - 2008


The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.


© Humana Press, a part of Springer Science+Business Media, LLC 2008

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