University of Hertfordshire

From the same journal

By the same authors

Red cell PMVs, plasma membrane-derived vesicles calling out for standards

Research output: Contribution to journalReview article

  • Elliott Hind
  • Sheelagh Heugh
  • Ephraim A Ansa-Addo
  • Samuel Antwi-Baffour
  • Sigrun Lange
  • Jameel Inal
View graph of relations
Original languageEnglish
Number of pages5
Pages (from-to)465-9
JournalBiochemical and Biophysical Research Communications
Journal publication date3 Sep 2010
Volume399
Issue4
Early online date30 Jul 2010
DOIs
Publication statusPublished - 3 Sep 2010

Abstract

Plasma membrane-derived vesicles (PMVs) or microparticles are vesicles (0.1-1mum in diameter) released from the plasma membrane of all blood cell types under a variety of biochemical and pathological conditions. PMVs contain cytoskeletal elements and some surface markers from the parent cell but lack a nucleus and are unable to synthesise macromolecules. They are also defined on the basis that in most cases PMVs express varying amounts of the cytosolic leaflet lipid phosphatidylserine, which is externalised during activation on their surface. This marks the PMV as a biologically distinct entity from that of its parent cell, despite containing surface markers from the original cell, and also explains its role in events such as phagocytosis and thrombosis. There is currently a large amount of variation between investigators with regard to the pre-analytical steps employed in isolating red cell PMVs or RPMVs (which are slightly smaller than most PMVs), with key differences being centrifugation and sample storage conditions, which often leads to result variability. Unfortunately, standardization of preparation and detection methods has not yet been achieved. This review highlights and critically discusses the variables contributing to differences in results obtained by investigators, bringing to light numerous studies of which RPMVs have been analysed but have not yet been the subject of a review.

ID: 13069480