University of Hertfordshire

Rotational-pulse actuated dissolvable-film valves for automated purification of total RNA from E. Coli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

  • D. J. Kinahan
  • E. Clancy
  • N. A. Kilcawley
  • N. Dimov
  • T. J. Smith
  • J. Ducrée
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Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1437-1439
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period26/10/1430/10/14

Abstract

In this work we report for the first time on a repertoire of valving technologies which are combined to enable automated purification of total RNA from cell homogenate. Process control is implemented using rotational-pulse actuated dissolvable-film (DF) valves; where the order of valve actuation is determined by the disc architecture while the timing of valve actuation is governed by pulses in the spin rate. Selective liquid routing is enabled by combining a heavy, inert and immiscible liquid plug with a DF. The combination of these technologies enables bead-based extraction of amplifiable RNA, with a yield comparable to gold-standard bench-top protocols.

ID: 13220287