University of Hertfordshire

Documents

  • A. R. Baydoun
  • G.E. Mann
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Original languageEnglish
Pages (from-to)726-731
JournalBiochemical and Biophysical Research Communications
Volume200
DOIs
Publication statusPublished - 1994

Abstract

Amino acid transport systems mediating uptake of nitric oxide (NO) synthase inhibitors were characterized in the murine macrophage cell line J774. Treatment of J774 cells with bacterial endotoxin (LPS, 1 μg ml-1, 24 h) selectively increased the transport capacity for NG-monomethyl-L-[14C]arginine (L-NMMA), whereas transport of NG-nitro-L-[3H]arginine (LNNA) was unaffected. Inhibition studies established that the cationic transport system y+ mediates uptake of L-arginine, L-NMMA and NG-iminoethyl-L-ornithine (L-NIO). A neutral transporter, with low substrate specificity and insensitive to LPS, mediates uptake of L-citrulline, L-NNA and its methyl ester L-NAME. We conclude that enhanced expression of the y+ transporter in LPS-stimulated macrophages (1) may facilitate the targeting of selective inhibitors of inducible NO synthase to activated cells generating NO in endotoxin shock.

Notes

Original article can be found at: http://www.sciencedirect.com/science/journal/0006291X Copyright Elsevier Inc. DOI : 10.1006/bbrc.1994.1511

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