University of Hertfordshire

Silica passivated conjugated polymer nanoparticles for biological imaging applications

Research output: Chapter in Book/Report/Conference proceedingChapter

Standard

Silica passivated conjugated polymer nanoparticles for biological imaging applications. / Bourke, Struan; Urbano, Laura; Olona, Antoni; Valderrama, Ferran; Ann Dailey, Lea; Green, Mark A.

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. Vol. 10079 SPIE, 2017.

Research output: Chapter in Book/Report/Conference proceedingChapter

Harvard

Bourke, S, Urbano, L, Olona, A, Valderrama, F, Ann Dailey, L & Green, MA 2017, Silica passivated conjugated polymer nanoparticles for biological imaging applications. in Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. vol. 10079, SPIE. https://doi.org/10.1117/12.2252035

APA

Bourke, S., Urbano, L., Olona, A., Valderrama, F., Ann Dailey, L., & Green, M. A. (2017). Silica passivated conjugated polymer nanoparticles for biological imaging applications. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX (Vol. 10079). SPIE. https://doi.org/10.1117/12.2252035

Vancouver

Bourke S, Urbano L, Olona A, Valderrama F, Ann Dailey L, Green MA. Silica passivated conjugated polymer nanoparticles for biological imaging applications. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. Vol. 10079. SPIE. 2017 https://doi.org/10.1117/12.2252035

Author

Bourke, Struan ; Urbano, Laura ; Olona, Antoni ; Valderrama, Ferran ; Ann Dailey, Lea ; Green, Mark A. / Silica passivated conjugated polymer nanoparticles for biological imaging applications. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. Vol. 10079 SPIE, 2017.

Bibtex

@inbook{3d1c3ce957434cc9b664377e2a8ebdb2,
title = "Silica passivated conjugated polymer nanoparticles for biological imaging applications",
abstract = "Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.",
keywords = "Biological imaging, Cancer imaging, Conjugated polymers, Nanoparticles, Silica shells",
author = "Struan Bourke and Laura Urbano and Antoni Olona and Ferran Valderrama and {Ann Dailey}, Lea and Green, {Mark A.}",
note = "{\circledC} 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).",
year = "2017",
month = "2",
day = "21",
doi = "10.1117/12.2252035",
language = "English",
volume = "10079",
booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX",
publisher = "SPIE",
address = "United States",

}

RIS

TY - CHAP

T1 - Silica passivated conjugated polymer nanoparticles for biological imaging applications

AU - Bourke, Struan

AU - Urbano, Laura

AU - Olona, Antoni

AU - Valderrama, Ferran

AU - Ann Dailey, Lea

AU - Green, Mark A.

N1 - © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).

PY - 2017/2/21

Y1 - 2017/2/21

N2 - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

AB - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

KW - Biological imaging, Cancer imaging, Conjugated polymers, Nanoparticles, Silica shells

U2 - 10.1117/12.2252035

DO - 10.1117/12.2252035

M3 - Chapter

VL - 10079

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX

PB - SPIE

ER -