University of Hertfordshire

  • Hassan H. Hammud
  • Kamal H. Bouhadir
  • Mamdouh S. Masoud
  • Amer M. Ghannoum
  • Sulaf A. Assi
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Original languageEnglish
Pages (from-to)895-917
Number of pages23
JournalJournal of Solution Chemistry
Volume37
Issue7
DOIs
Publication statusPublished - Jul 2008

Abstract

The absorption and emission spectra of six purine derivatives: adenine (I), N(9)-hydroxyethyladenine (II), N(6)-acetyladenine (III), N(6)-isobutyryladenine (IV), guanine (V), and N(2),N(9)-diacetylguanine (VI) have been investigated. The effects of solvent and pH on the positions of lambda (max) (absorption) and lambda (max) (emission) of these compounds were determined. Correlations between the absorption wavelength (lambda (max)) of these organic compounds and the solvent parameters (D,n,E) or (K,M,N) show that the peak position is affected mainly by specific- and non-specific types of interactions between the solvent and solute. Solvent effects on the electronic absorption band shifts are indicative of the extent of charge reorganization of the solute molecules upon electronic excitation. The Stokes shift (nu (abs)-nu (em)) was correlated with the orientation polarizability (Delta f) and was found to depend mainly on the dielectric constant and the refractive index of the solvents. This shift reflects the influence of the equilibrium solvent arrangement around the excited solute molecule, which rearranges inertially due to the instantaneous charge redistribution upon radiative deactivation to the electronic ground state. A spectrofluorometric analysis technique was applied for the quantitative analysis of the components of a ternary mixture of compounds (I-III).

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