University of Hertfordshire

From the same journal

By the same authors

Documents

  • Charlotte Hall
  • Helen Lydon
  • Christopher Dalton
  • J. Kevin Chipman
  • John Graham
  • Robert Chilcott
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Original languageEnglish
Number of pages10
Pages (from-to)1036-1045
JournalJournal of Applied Toxicology
Journal publication date18 Jul 2017
Volume37
Issue9
Early online date17 Mar 2017
DOIs
Publication statusPublished - 18 Jul 2017

Abstract

This study used a damaged skin, porcine model to evaluate the in vivo efficacy of WoundStat™ for decontamination of
superficial (non-haemorrhaging), sulphur mustard-contaminated wounds. The dorsal skin of 12 female pigs was subjected to controlled
physical damage and exposed to 10 μL 14C–radiolabelled sulphur mustard (14C–SM). Animals were randomly assigned to
either a control or a treatment group. In the latter, WoundStat™ was applied 30 s post exposure and left in situ for 1 h. Skin lesion
progression and decontaminant efficacy were quantified over 6 h using a range of biophysical measurements. Skin, blood and
organ samples were taken post mortem for histopathological assessment, 14C–SM distribution and toxicokinetic analyses. Application
of SM to damaged skin without decontamination was rapidly followed by advanced signs of toxicity, including ulceration
and decreased blood flow at the exposure site in all animals. WoundStat™ prevented ulceration and improved blood flow at the
exposure site in all decontaminated animals (n = 6). Furthermore, significantly smaller quantities of 14C–SM were detected in the
blood (45% reduction), and recovered from skin (70% reduction) and skin surface swabs (99% reduction) at 6 h post-challenge.
Overall, the distribution of 14C–SM in the internal organs was similar for both groups, with the greatest concentration in the kidneys,
followed by the liver and small intestine. WoundStat™ significantly reduced the amount of 14C–SM recovered from the liver,
a key organ for SM metabolism and detoxification. This study demonstrates that WoundStat™ is a suitable product for reducing
the ingress and toxicity of a chemical warfare agent.

Notes

This is the peer reviewed version of the following article: Charlotte A. Hall, et al, 'The percutaneous toxicokinetics of Sulphur mustard in a damaged skin porcine model and the evaluation of WoundStat™ as a topical decontaminant', Journal of Applied Toxicology, July 2017, which has been published in final form at DOI: 10.1002/jat.3453. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Copyright © 2017 John Wiley & Sons, Ltd.

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