φ20, a temperate bacteriophage isolated from Bacillus anthracis exists as a plasmidial prophage

Jameel M. Inal, Karuna V. Karunakaran

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22 Citations (Scopus)

Abstract

This study describes the isolation of temperate B. anthracis phages, from 4 out of 20 B. anthracis strains screened, by use of the inducing agents mitomycin C and UV light. Phage φ20 isolated from B. anthracis Sterne 34F2 (pXO1+ pXO2-) was shown to have double-stranded DNA of size 48756 bp and a restriction site map showing nine sites for enzymes BamHI, BglII, and SstI is included. The φ20 genome was found to exist as a plasmidial prophage and the phage itself to have a polyhedral head of diameter 65 nm and tail 217 nm long and 15 nm wide.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalCurrent Microbiology
Volume32
Issue number4
DOIs
Publication statusPublished - Apr 1996

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