A hyperspectral fluorescence lifetime probe for skin cancer diagnosis

P A A De Beule, C Dunsby, N P Galletly, G W Stamp, A C Chu, U Anand, P Anand, C.D. Benham, A Naylor, P M W French

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


The autofluorescence of biological tissue can be exploited for the detection and diagnosis of disease but, to date, its complex nature and relatively weak signal levels have impeded its widespread application in biology and medicine. We present here a portable instrument designed for the in situ simultaneous measurement of autofluorescence emission spectra and temporal decay profiles, permitting the analysis of complex fluorescence signals. This hyperspectral fluorescence lifetime probe utilizes two ultrafast lasers operating at 355 and 440 nm that can excite autofluorescence from many different biomolecules present in skin tissue including keratin, collagen, nicotinamide adenine dinucleotide (phosphate), and flavins. The instrument incorporates an optical fiber probe to provide sample illumination and fluorescence collection over a millimeter-sized area. We present a description of the system, including spectral and temporal characterizations, and report the preliminary application of this instrument to a study of recently resected (
Original languageEnglish
Article number123101
JournalReview of scientific instruments
Issue number12
Publication statusPublished - 2007


  • Equipment Design
  • Equipment Failure Analysis
  • Humans
  • Luminescent Measurements
  • Molecular Probe Techniques
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Skin Neoplasms
  • Spectrometry, Fluorescence
  • Tumor Markers, Biological


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