A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Angela  Van de Wouw; Jack  Scanlan; Hawlader  Al-Mamun; Marie-Hélène  Balesdent; Lydia   Bousset; Lenka   Burketová; Luis   del Rio Mendoza; Dilantha   Fernando; Coreen  Franke; Barbara   Howlett; Yongju Huang; Eirian  Jones; Birger   Koopmann; Suhaizan  Lob; Ali  Mirabadi; Bridgit   Nugent; Gary  Peng; Franco   Rossi; Huibrecht  Schreuder; Annie  Tabone; Gert   Van Coller; Jacqueline   Batley; Alexander   Idnurm

doi:10.1111/ppa.13801

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Angela Van de Wouw, Jack Scanlan, Hawlader Al-Mamun, Marie-Hélène Balesdent, Lydia Bousset, Lenka Burketová, Luis del Rio Mendoza, Dilantha Fernando, Coreen Franke, Barbara Howlett, Yongju Huang, Eirian Jones, Birger Koopmann, Suhaizan Lob, Ali Mirabadi, Bridgit Nugent, Gary Peng, Franco Rossi, Huibrecht Schreuder, Annie TaboneGert Van Coller, Jacqueline Batley, Alexander Idnurm

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Abstract

A collection of isolates of the fungi Leptosphaeria maculans and L. biglobosa, which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L. maculans and 21 L. biglobosa) from 11 countries. The genomes of all 205 L. maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B. napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single-nucleotide polymorphism (SNP) diversity within these L. maculans isolates revealed geographical separation of the populations. This "open access" resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.

Original language	English
Journal	Plant Pathology
Early online date	22 Sept 2023
DOIs	https://doi.org/10.1111/ppa.13801
Publication status	E-pub ahead of print - 22 Sept 2023

Keywords

avirulence gene
blackleg
canola
culture collection
oilseed rape

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10.1111/ppa.13801Licence: CC BY-NC-ND

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© 2023 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. This is an open access article under the terms of the Creative Commons Attribution-Non Commercial-No Derivs License. https://creativecommons.org/licenses/by-nc-nd/4.0/
Final published version, 4.94 MBLicence: CC BY-NC-ND

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Van de Wouw, A., Scanlan, J., Al-Mamun, H., Balesdent, M.-H., Bousset, L., Burketová, L., del Rio Mendoza, L., Fernando, D., Franke, C., Howlett, B., Huang, Y., Jones, E., Koopmann, B., Lob, S., Mirabadi, A., Nugent, B., Peng, G., Rossi, F., Schreuder, H., ... Idnurm, A. (2023). A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus. Plant Pathology. Advance online publication. https://doi.org/10.1111/ppa.13801

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abstract = "A collection of isolates of the fungi Leptosphaeria maculans and L. biglobosa, which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L. maculans and 21 L. biglobosa) from 11 countries. The genomes of all 205 L. maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B. napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single-nucleotide polymorphism (SNP) diversity within these L. maculans isolates revealed geographical separation of the populations. This {"}open access{"} resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.",

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Van de Wouw, A, Scanlan, J, Al-Mamun, H, Balesdent, M-H, Bousset, L, Burketová, L, del Rio Mendoza, L, Fernando, D, Franke, C, Howlett, B, Huang, Y, Jones, E, Koopmann, B, Lob, S, Mirabadi, A, Nugent, B, Peng, G, Rossi, F, Schreuder, H, Tabone, A, Van Coller, G, Batley, J & Idnurm, A 2023, 'A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus', Plant Pathology. https://doi.org/10.1111/ppa.13801

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AU - Van de Wouw, Angela

AU - Scanlan, Jack

AU - Al-Mamun, Hawlader

AU - Balesdent, Marie-Hélène

AU - Bousset, Lydia

AU - Burketová, Lenka

AU - del Rio Mendoza, Luis

AU - Fernando, Dilantha

AU - Franke, Coreen

AU - Howlett, Barbara

AU - Huang, Yongju

AU - Jones, Eirian

AU - Koopmann, Birger

AU - Lob, Suhaizan

AU - Mirabadi, Ali

AU - Nugent, Bridgit

AU - Peng, Gary

AU - Rossi, Franco

AU - Schreuder, Huibrecht

AU - Tabone, Annie

AU - Van Coller, Gert

AU - Batley, Jacqueline

AU - Idnurm, Alexander

N1 - © 2023 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. This is an open access article under the terms of the Creative Commons Attribution-Non Commercial-No Derivs License. https://creativecommons.org/licenses/by-nc-nd/4.0/

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N2 - A collection of isolates of the fungi Leptosphaeria maculans and L. biglobosa, which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L. maculans and 21 L. biglobosa) from 11 countries. The genomes of all 205 L. maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B. napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single-nucleotide polymorphism (SNP) diversity within these L. maculans isolates revealed geographical separation of the populations. This "open access" resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.

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KW - avirulence gene

KW - blackleg

KW - canola

KW - culture collection

KW - oilseed rape

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DO - 10.1111/ppa.13801

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SN - 0032-0862

JO - Plant Pathology

JF - Plant Pathology

ER -

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

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Keywords

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