A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Angela Van de Wouw, Jack Scanlan, Hawlader Al-Mamun, Marie-Hélène Balesdent, Lydia Bousset, Lenka Burketová, Luis del Rio Mendoza, Dilantha Fernando, Coreen Franke, Barbara Howlett, Yongju Huang, Eirian Jones, Birger Koopmann, Suhaizan Lob, Ali Mirabadi, Bridgit Nugent, Gary Peng, Franco Rossi, Huibrecht Schreuder, Annie TaboneGert Van Coller, Jacqueline Batley, Alexander Idnurm

Research output: Contribution to journalArticlepeer-review

9 Downloads (Pure)

Abstract

A collection of isolates of the fungi Leptosphaeria maculans and L. biglobosa, which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L. maculans and 21 L. biglobosa) from 11 countries. The genomes of all 205 L. maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B. napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single-nucleotide polymorphism (SNP) diversity within these L. maculans isolates revealed geographical separation of the populations. This "open access" resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.
Original languageEnglish
JournalPlant Pathology
Early online date22 Sept 2023
DOIs
Publication statusE-pub ahead of print - 22 Sept 2023

Keywords

  • avirulence gene
  • blackleg
  • canola
  • culture collection
  • oilseed rape

Fingerprint

Dive into the research topics of 'A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus'. Together they form a unique fingerprint.

Cite this