TY - JOUR
T1 - A novel treatment strategy for preterm birth: Intra-vaginal progesterone-loaded fibrous patches
AU - Emin Cam, Muhammet
AU - Nur Hazar-Yavuz, Ayse
AU - Cesur, Sumeyye
AU - Ozkan, Ozan
AU - Alenezi, Hussain
AU - Turkoglu Sasmazel, Hilal
AU - Sayip Eroglu, Mehmet
AU - Brako, Francis
AU - Ahmed, Jubair
AU - Kabasakal, Levent
AU - Ren, Guogang
AU - Gunduz, Oguzhan
AU - Edirisinghe, Mohan
N1 - © 2020 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - Progesterone-loaded poly(lactic) acid fibrous polymeric patches were produced using electrospinning and pressurized gyration for intra-vaginal application to prevent preterm birth. The patches were intravaginally inserted into rats in the final week of their pregnancy, equivalent to the third trimester of human pregnancy. Maintenance tocolysis with progesterone-loaded patches was elucidated by recording the contractile response of uterine smooth muscle to noradrenaline in pregnant rats. Both progesterone-loaded patches indicated similar results from release and thermal studies, however, patches obtained by electrospinning had smaller average diameters and more uniform dispersion compared to pressurized gyration. Patches obtained by pressurized gyration had better results in production yield and tensile strength than electrospinning; thereby pressurized gyration is better suited for scaled-up production. The patches did not affect cell attachment, viability, and proliferation on Vero cells negatively. Consequently, progesterone-loaded patches are a novel and successful treatment strategy for preventing preterm birth.
AB - Progesterone-loaded poly(lactic) acid fibrous polymeric patches were produced using electrospinning and pressurized gyration for intra-vaginal application to prevent preterm birth. The patches were intravaginally inserted into rats in the final week of their pregnancy, equivalent to the third trimester of human pregnancy. Maintenance tocolysis with progesterone-loaded patches was elucidated by recording the contractile response of uterine smooth muscle to noradrenaline in pregnant rats. Both progesterone-loaded patches indicated similar results from release and thermal studies, however, patches obtained by electrospinning had smaller average diameters and more uniform dispersion compared to pressurized gyration. Patches obtained by pressurized gyration had better results in production yield and tensile strength than electrospinning; thereby pressurized gyration is better suited for scaled-up production. The patches did not affect cell attachment, viability, and proliferation on Vero cells negatively. Consequently, progesterone-loaded patches are a novel and successful treatment strategy for preventing preterm birth.
KW - Electrospinning
KW - Fibers
KW - Polymeric patch
KW - Pressurized gyration
KW - Preterm birth
KW - Progesterone
UR - http://www.scopus.com/inward/record.url?scp=85089830531&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2020.119782
DO - 10.1016/j.ijpharm.2020.119782
M3 - Article
C2 - 32822780
SN - 0378-5173
VL - 588
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 119782
ER -