A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots

Euphemia Mutasa-Gottgens; Elaine  Ward; M. J.  Adams; C. R.  Collier; D. M. Chwarszynska; M. J. C. Asher

doi:10.1006/pmpp.1993.1066

A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots

Euphemia Mutasa-Gottgens, Elaine Ward, M. J. Adams, C. R. Collier, D. M. Chwarszynska, M. J. C. Asher

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment detected four isolates of P. betae in infected roots and did not cross hybridize with DNA from healthy sugar beet, contaminating microorganisms commonly found within the sugar beet rhizosphere, or isolates of the closely related Polymyxa graminis and Olpidium brassicae. This probe provides a molecular detection method for P. betae which is quicker and likely to be more sensitive than conventional microscopic examination

Original language	English
Pages (from-to)	379-390
Number of pages	12
Journal	Physiological and Molecular Plant Pathology
Volume	43
Issue number	5
DOIs	https://doi.org/10.1006/pmpp.1993.1066
Publication status	Published - Nov 1993

Access to Document

10.1006/pmpp.1993.1066

Cite this

@article{c2539aa8b2ae438db7c81a9e2a19e922,

title = "A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots",

abstract = "A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment detected four isolates of P. betae in infected roots and did not cross hybridize with DNA from healthy sugar beet, contaminating microorganisms commonly found within the sugar beet rhizosphere, or isolates of the closely related Polymyxa graminis and Olpidium brassicae. This probe provides a molecular detection method for P. betae which is quicker and likely to be more sensitive than conventional microscopic examination",

author = "Euphemia Mutasa-Gottgens and Elaine Ward and Adams, {M. J.} and Collier, {C. R.} and Chwarszynska, {D. M.} and Asher, {M. J. C.}",

year = "1993",

month = nov,

doi = "10.1006/pmpp.1993.1066",

language = "English",

volume = "43",

pages = "379--390",

journal = "Physiological and Molecular Plant Pathology",

issn = "0885-5765",

publisher = "Academic Press Inc.",

number = "5",

}

TY - JOUR

T1 - A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots

AU - Mutasa-Gottgens, Euphemia

AU - Ward, Elaine

AU - Adams, M. J.

AU - Collier, C. R.

AU - Chwarszynska, D. M.

AU - Asher, M. J. C.

PY - 1993/11

Y1 - 1993/11

N2 - A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment detected four isolates of P. betae in infected roots and did not cross hybridize with DNA from healthy sugar beet, contaminating microorganisms commonly found within the sugar beet rhizosphere, or isolates of the closely related Polymyxa graminis and Olpidium brassicae. This probe provides a molecular detection method for P. betae which is quicker and likely to be more sensitive than conventional microscopic examination

AB - A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment detected four isolates of P. betae in infected roots and did not cross hybridize with DNA from healthy sugar beet, contaminating microorganisms commonly found within the sugar beet rhizosphere, or isolates of the closely related Polymyxa graminis and Olpidium brassicae. This probe provides a molecular detection method for P. betae which is quicker and likely to be more sensitive than conventional microscopic examination

U2 - 10.1006/pmpp.1993.1066

DO - 10.1006/pmpp.1993.1066

M3 - Article

SN - 0885-5765

VL - 43

SP - 379

EP - 390

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

IS - 5

ER -

A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots

Abstract

Access to Document

Fingerprint

Cite this