A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens

Colin Malcolm; D. Bourguet; A. Ascolillo; S. J. Rooker; C. F. Garvey; L. M. Hall; N. Pasteur; M. Raymond

doi:10.1046/j.1365-2583.1998.72055.x

A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens

Colin Malcolm, D. Bourguet, A. Ascolillo, S. J. Rooker, C. F. Garvey, L. M. Hall, N. Pasteur, M. Raymond

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.

Original language	English
Pages (from-to)	107-120
Number of pages	14
Journal	Insect Molecular Biology
Volume	7
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2583.1998.72055.x
Publication status	Published - May 1998

Keywords

Acetylcholinesterase
Amino Acid Sequence
Animals
Base Sequence
Culex
DNA, Complementary
Female
Humans
Insecticide Resistance
Male
Molecular Sequence Data
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sex Factors

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10.1046/j.1365-2583.1998.72055.x

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title = "A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens",

abstract = "An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.",

keywords = "Acetylcholinesterase, Amino Acid Sequence, Animals, Base Sequence, Culex, DNA, Complementary, Female, Humans, Insecticide Resistance, Male, Molecular Sequence Data, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sex Factors",

author = "Colin Malcolm and D. Bourguet and A. Ascolillo and Rooker, {S. J.} and Garvey, {C. F.} and Hall, {L. M.} and N. Pasteur and M. Raymond",

year = "1998",

month = may,

doi = "10.1046/j.1365-2583.1998.72055.x",

language = "English",

volume = "7",

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journal = "Insect Molecular Biology",

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T1 - A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens

AU - Malcolm, Colin

AU - Bourguet, D.

AU - Ascolillo, A.

AU - Rooker, S. J.

AU - Garvey, C. F.

AU - Hall, L. M.

AU - Pasteur, N.

AU - Raymond, M.

PY - 1998/5

Y1 - 1998/5

N2 - An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.

AB - An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.

KW - Acetylcholinesterase

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Culex

KW - DNA, Complementary

KW - Female

KW - Humans

KW - Insecticide Resistance

KW - Male

KW - Molecular Sequence Data

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Sex Factors

U2 - 10.1046/j.1365-2583.1998.72055.x

DO - 10.1046/j.1365-2583.1998.72055.x

M3 - Article

C2 - 9535157

SN - 0962-1075

VL - 7

SP - 107

EP - 120

JO - Insect Molecular Biology

JF - Insect Molecular Biology

IS - 2

ER -

A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens

Abstract

Keywords

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