A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses

Cecilia Lindau, Annika Tiveljung-Lindell, Shan Goh, Torbjörn Ramqvist, Tobias Allander

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

BACKGROUND: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.

OBJECTIVES: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.

STUDY DESIGN: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.

RESULTS: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.

CONCLUSIONS: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.

Original languageEnglish
Pages (from-to)24-6
Number of pages3
JournalJournal of Clinical Virology
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2009

Keywords

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Nasopharynx
  • Polymerase Chain Reaction
  • Polyomavirus
  • Polyomavirus Infections
  • Sensitivity and Specificity
  • Tumor Virus Infections
  • Young Adult
  • Evaluation Studies
  • Journal Article
  • Research Support, Non-U.S. Gov't

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