Abstract
BACKGROUND: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.
OBJECTIVES: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.
STUDY DESIGN: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.
RESULTS: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.
CONCLUSIONS: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
Original language | English |
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Pages (from-to) | 24-6 |
Number of pages | 3 |
Journal | Journal of Clinical Virology |
Volume | 44 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2009 |
Keywords
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Child
- Child, Preschool
- Female
- Humans
- Infant
- Infant, Newborn
- Male
- Middle Aged
- Nasopharynx
- Polymerase Chain Reaction
- Polyomavirus
- Polyomavirus Infections
- Sensitivity and Specificity
- Tumor Virus Infections
- Young Adult
- Evaluation Studies
- Journal Article
- Research Support, Non-U.S. Gov't