BACKGROUND: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.
OBJECTIVES: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.
STUDY DESIGN: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.
RESULTS: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.
CONCLUSIONS: The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
- Aged, 80 and over
- Child, Preschool
- Infant, Newborn
- Middle Aged
- Polymerase Chain Reaction
- Polyomavirus Infections
- Sensitivity and Specificity
- Tumor Virus Infections
- Young Adult
- Evaluation Studies
- Journal Article
- Research Support, Non-U.S. Gov't