AfuPmV-1-Infected Aspergillus fumigatus Is More Susceptible to Stress Than Virus-Free Fungus

Gabriele Sass, Marife Martinez, Ioly Kotta-Loizou, David Stevens, Isabelle Mouyna (Editor)

Research output: Contribution to journalArticlepeer-review

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Abstract

Infection with Aspergillus fumigatus polymycovirus 1 (AfuPmV-1) affects Aspergillus fumigatus Af293’s growth in vitro, iron metabolism, resistance in intermicrobial competition with Pseudomonas aeruginosa, resistance to osmotic stress, and resistance to the chitin synthase inhibitor nikkomycin Z. Here, we show that response to high temperature, Congo Red-induced stress, and hydrogen peroxide are also dependent on the viral infection status of A. fumigatus. AfuPmV-1- infected Af293 was more susceptible than virus-free Af293 to growth inhibition by high temperature, hydrogen peroxide, Congo Red exposure, and nutrient restriction. Increased resistance of virus-free fungus was observed when cultures were started from conidia but, in the case of high temperature and hydrogen peroxide, not when cultures were started from hyphae. This indicates that the virus impairs the stress response during the growth phase of germination of conidia and development into hyphae. In conclusion, our work indicates that AfuPmV-1 infection in A. fumigatus impairs host responses to stress, as shown by exposure to high temperature, oxidative stress such as hydrogen peroxide, and some cell wall stresses, as shown by exposure to Congo Red (in agreement with our previous observations using nikkomycin Z) and nutrient restriction.
Original languageEnglish
Article number750
Pages (from-to)1-17
Number of pages17
JournalJournal of Fungi (JoF)
Volume9
Issue number7
Early online date15 Jul 2023
DOIs
Publication statusE-pub ahead of print - 15 Jul 2023

Keywords

  • fungal growth
  • polymycovirus
  • oxidative stress
  • cell wall stress
  • temperature stress
  • Aspergillus fumigatus

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