Agarose gel electrophoresis of nucleic acids

D.R Williams; Ralph Rapley

doi:10.1385/1-59259-038-1:67

Agarose gel electrophoresis of nucleic acids

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs

Original language	English
Title of host publication	The Nucleic Acids Protocols Handbook
Editors	R Rapley
Place of Publication	Totowa, NJ
Publisher	Humana Press
Pages	67-70
Number of pages	4
Edition	Part II
ISBN (Electronic)	978-1-59259-038-4
ISBN (Print)	978-0-89603-459-4
DOIs	https://doi.org/10.1385/1-59259-038-1:67
Publication status	Published - 2000

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10.1385/1-59259-038-1:67

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title = "Agarose gel electrophoresis of nucleic acids",

abstract = "DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs",

author = "D.R Williams and Ralph Rapley",

note = "D. R. Williams and R. Rapley, 'Agarose gel electrophoresis of nucleic acids' in Ralph Rapley, ed., The Nucleic Acid Protocols Handbook (Totowa: Humana Press, 2000) ISBN: 978-0-89603-459-4, eISBN: 978-1-59259-038-4, DOI: 10.1385/1-59259-038-1:67 {\textcopyright} 2000 Humana Press Inc., Totowa, NJ.",

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booktitle = "The Nucleic Acids Protocols Handbook",

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edition = "Part II",

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T1 - Agarose gel electrophoresis of nucleic acids

AU - Williams, D.R

AU - Rapley, Ralph

N1 - D. R. Williams and R. Rapley, 'Agarose gel electrophoresis of nucleic acids' in Ralph Rapley, ed., The Nucleic Acid Protocols Handbook (Totowa: Humana Press, 2000) ISBN: 978-0-89603-459-4, eISBN: 978-1-59259-038-4, DOI: 10.1385/1-59259-038-1:67 © 2000 Humana Press Inc., Totowa, NJ.

PY - 2000

Y1 - 2000

N2 - DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs

AB - DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs

UR - https://link.springer.com/protocol/10.1385/1-59259-038-1%3A67

U2 - 10.1385/1-59259-038-1:67

DO - 10.1385/1-59259-038-1:67

M3 - Chapter (peer-reviewed)

SN - 978-0-89603-459-4

SP - 67

EP - 70

BT - The Nucleic Acids Protocols Handbook

A2 - Rapley, R

PB - Humana Press

CY - Totowa, NJ

ER -

Agarose gel electrophoresis of nucleic acids

Abstract

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