An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi

Thomas Wilkes, Douglas Warner

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Abstract

Sheaffer blue ink is an effective method to stain arbuscular mycorrhizal (AM) fungi in a variety of plant species. It has, however, received criticism for its potential rapid degradation and short-term viability. The long and medium term storage and viability of stained samples has not, to date, been described for this particular staining method. This short communication reports on the viability of 730 samples stained with Sheaffer® blue ink stored for the duration of four years in microscope slide boxes out of direct sunlight. There was no significant difference in micrograph image quality and presence of stain between years as indicated by the number of AM fungal structures quantified. In conclusion Sheaffer® blue ink stain does not deteriorate in the medium term.
Original languageEnglish
Article number000292
Number of pages4
JournalAccess Microbiology
Volume3
Issue number11
Early online date30 Nov 2021
DOIs
Publication statusPublished - 30 Nov 2021

Keywords

  • arbuscular mycorrhizal fungi
  • blue ink stain
  • root cortex
  • storage
  • degradation

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