An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue

Patrick F Sullivan; Tobias Allander; Fredrik Lysholm; Shan Goh; Bengt Persson; Andreas Jacks; Birgitta Evengård; Nancy L. Pedersen; Björn Andersson

doi:10.1186/1471-2180-11-2

An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue

Patrick F Sullivan, Tobias Allander, Fredrik Lysholm, Shan Goh, Bengt Persson, Andreas Jacks, Birgitta Evengård, Nancy L. Pedersen, Björn Andersson

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

BACKGROUND: Chronic fatigue syndrome is an idiopathic syndrome widely suspected of having an infectious or immune etiology. We applied an unbiased metagenomic approach to try to identify known or novel infectious agents in the serum of 45 cases with chronic fatigue syndrome or idiopathic chronic fatigue. Controls were the unaffected monozygotic co-twins of cases, and serum samples were obtained at the same place and time.

RESULTS: No novel DNA or RNA viral signatures were confidently identified. Four affected twins and no unaffected twins evidenced viremia with GB virus C (8.9% vs. 0%, p = 0.019), and one affected twin had previously undetected hepatitis C viremia. An excess of GB virus C viremia in cases with chronic fatigue requires confirmation.

CONCLUSIONS: Current, impairing chronic fatigue was not robustly associated with viremia detectable in serum.

Original language	English
Pages (from-to)	2
Journal	BMC Microbiology
Volume	11
DOIs	https://doi.org/10.1186/1471-2180-11-2
Publication status	Published - 2 Jan 2011

Keywords

Adult
Communicable Diseases
Cross-Sectional Studies
DNA, Viral
Diseases in Twins
Fatigue Syndrome, Chronic
Female
GB virus C
Humans
Male
Metagenomics
Middle Aged
RNA, Viral
Risk Factors
Twins, Monozygotic
Viremia
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Twin Study

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10.1186/1471-2180-11-2

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title = "An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue",

abstract = "BACKGROUND: Chronic fatigue syndrome is an idiopathic syndrome widely suspected of having an infectious or immune etiology. We applied an unbiased metagenomic approach to try to identify known or novel infectious agents in the serum of 45 cases with chronic fatigue syndrome or idiopathic chronic fatigue. Controls were the unaffected monozygotic co-twins of cases, and serum samples were obtained at the same place and time.RESULTS: No novel DNA or RNA viral signatures were confidently identified. Four affected twins and no unaffected twins evidenced viremia with GB virus C (8.9% vs. 0%, p = 0.019), and one affected twin had previously undetected hepatitis C viremia. An excess of GB virus C viremia in cases with chronic fatigue requires confirmation.CONCLUSIONS: Current, impairing chronic fatigue was not robustly associated with viremia detectable in serum.",

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author = "Sullivan, {Patrick F} and Tobias Allander and Fredrik Lysholm and Shan Goh and Bengt Persson and Andreas Jacks and Birgitta Eveng{\aa}rd and Pedersen, {Nancy L.} and Bj{\"o}rn Andersson",

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doi = "10.1186/1471-2180-11-2",

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T1 - An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue

AU - Sullivan, Patrick F

AU - Allander, Tobias

AU - Lysholm, Fredrik

AU - Goh, Shan

AU - Persson, Bengt

AU - Jacks, Andreas

AU - Evengård, Birgitta

AU - Pedersen, Nancy L.

AU - Andersson, Björn

PY - 2011/1/2

Y1 - 2011/1/2

N2 - BACKGROUND: Chronic fatigue syndrome is an idiopathic syndrome widely suspected of having an infectious or immune etiology. We applied an unbiased metagenomic approach to try to identify known or novel infectious agents in the serum of 45 cases with chronic fatigue syndrome or idiopathic chronic fatigue. Controls were the unaffected monozygotic co-twins of cases, and serum samples were obtained at the same place and time.RESULTS: No novel DNA or RNA viral signatures were confidently identified. Four affected twins and no unaffected twins evidenced viremia with GB virus C (8.9% vs. 0%, p = 0.019), and one affected twin had previously undetected hepatitis C viremia. An excess of GB virus C viremia in cases with chronic fatigue requires confirmation.CONCLUSIONS: Current, impairing chronic fatigue was not robustly associated with viremia detectable in serum.

AB - BACKGROUND: Chronic fatigue syndrome is an idiopathic syndrome widely suspected of having an infectious or immune etiology. We applied an unbiased metagenomic approach to try to identify known or novel infectious agents in the serum of 45 cases with chronic fatigue syndrome or idiopathic chronic fatigue. Controls were the unaffected monozygotic co-twins of cases, and serum samples were obtained at the same place and time.RESULTS: No novel DNA or RNA viral signatures were confidently identified. Four affected twins and no unaffected twins evidenced viremia with GB virus C (8.9% vs. 0%, p = 0.019), and one affected twin had previously undetected hepatitis C viremia. An excess of GB virus C viremia in cases with chronic fatigue requires confirmation.CONCLUSIONS: Current, impairing chronic fatigue was not robustly associated with viremia detectable in serum.

KW - Adult

KW - Communicable Diseases

KW - Cross-Sectional Studies

KW - DNA, Viral

KW - Diseases in Twins

KW - Fatigue Syndrome, Chronic

KW - Female

KW - GB virus C

KW - Humans

KW - Male

KW - Metagenomics

KW - Middle Aged

KW - RNA, Viral

KW - Risk Factors

KW - Twins, Monozygotic

KW - Viremia

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Twin Study

U2 - 10.1186/1471-2180-11-2

DO - 10.1186/1471-2180-11-2

M3 - Article

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VL - 11

SP - 2

JO - BMC Microbiology

JF - BMC Microbiology

ER -

An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue

Abstract

Keywords

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