Antibacterial compounds of the cultivated Robusta coffee: Coffea canephora Pierre ex A. Froehner

Holly Siddique

doi:10.1016/j.sciaf.2022.e01274

Antibacterial compounds of the cultivated Robusta coffee: Coffea canephora Pierre ex A. Froehner

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Abstract

TThe stems and leaves of robusta coffee, Coffea canephora Pierre ex A. Froehner, one of the traded Coffea species, yielded caffeine (1), lupeol (2), ursolic acid (3), 3β-acetyl oleanolic acid (4), friedelin (5), asperphernamate (6), squalene (7), stigmasterol (8), stigmasterol-3-O-β-D-glucopyranoside (9) and chlorophyll a (10). The structures of the compounds were determined using 1D, 2D NMR experiments, IR and MS. Ursolic acid, 3β-acetyl oleanolic acid, caffeine and chlorophyll a, were studied for their antibacterial inhibition effects against methicillin resistant Staphylocccus aureus (MRSA) clinical strains SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819. Ursolic acid showed highest antibacterial activity against all the tested organisms with the MIC of 8–16 µg/ml. Ursolic acid showed 1-to-4-fold better activity when compared to norfloxacin against SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819.

Original language	English
Journal	Scientific African
Volume	16
DOIs	https://doi.org/10.1016/j.sciaf.2022.e01274
Publication status	Published - 9 Jul 2022

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title = "Antibacterial compounds of the cultivated Robusta coffee: Coffea canephora Pierre ex A. Froehner",

abstract = "TThe stems and leaves of robusta coffee, Coffea canephora Pierre ex A. Froehner, one of the traded Coffea species, yielded caffeine (1), lupeol (2), ursolic acid (3), 3β-acetyl oleanolic acid (4), friedelin (5), asperphernamate (6), squalene (7), stigmasterol (8), stigmasterol-3-O-β-D-glucopyranoside (9) and chlorophyll a (10). The structures of the compounds were determined using 1D, 2D NMR experiments, IR and MS. Ursolic acid, 3β-acetyl oleanolic acid, caffeine and chlorophyll a, were studied for their antibacterial inhibition effects against methicillin resistant Staphylocccus aureus (MRSA) clinical strains SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819. Ursolic acid showed highest antibacterial activity against all the tested organisms with the MIC of 8–16 µg/ml. Ursolic acid showed 1-to-4-fold better activity when compared to norfloxacin against SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819.",

author = "Holly Siddique",

year = "2022",

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language = "English",

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T1 - Antibacterial compounds of the cultivated Robusta coffee: Coffea canephora Pierre ex A. Froehner

AU - Siddique, Holly

PY - 2022/7/9

Y1 - 2022/7/9

N2 - TThe stems and leaves of robusta coffee, Coffea canephora Pierre ex A. Froehner, one of the traded Coffea species, yielded caffeine (1), lupeol (2), ursolic acid (3), 3β-acetyl oleanolic acid (4), friedelin (5), asperphernamate (6), squalene (7), stigmasterol (8), stigmasterol-3-O-β-D-glucopyranoside (9) and chlorophyll a (10). The structures of the compounds were determined using 1D, 2D NMR experiments, IR and MS. Ursolic acid, 3β-acetyl oleanolic acid, caffeine and chlorophyll a, were studied for their antibacterial inhibition effects against methicillin resistant Staphylocccus aureus (MRSA) clinical strains SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819. Ursolic acid showed highest antibacterial activity against all the tested organisms with the MIC of 8–16 µg/ml. Ursolic acid showed 1-to-4-fold better activity when compared to norfloxacin against SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819.

AB - TThe stems and leaves of robusta coffee, Coffea canephora Pierre ex A. Froehner, one of the traded Coffea species, yielded caffeine (1), lupeol (2), ursolic acid (3), 3β-acetyl oleanolic acid (4), friedelin (5), asperphernamate (6), squalene (7), stigmasterol (8), stigmasterol-3-O-β-D-glucopyranoside (9) and chlorophyll a (10). The structures of the compounds were determined using 1D, 2D NMR experiments, IR and MS. Ursolic acid, 3β-acetyl oleanolic acid, caffeine and chlorophyll a, were studied for their antibacterial inhibition effects against methicillin resistant Staphylocccus aureus (MRSA) clinical strains SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819. Ursolic acid showed highest antibacterial activity against all the tested organisms with the MIC of 8–16 µg/ml. Ursolic acid showed 1-to-4-fold better activity when compared to norfloxacin against SA1199B, XU212, ATCC25923, MRSA 340702 and MRSA 27819.

U2 - 10.1016/j.sciaf.2022.e01274

DO - 10.1016/j.sciaf.2022.e01274

M3 - Article

VL - 16

JO - Scientific African

JF - Scientific African

ER -

Antibacterial compounds of the cultivated Robusta coffee: Coffea canephora Pierre ex A. Froehner

Abstract

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