TY - JOUR
T1 - Application of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling
T2 - the Food4Me project
AU - Hoeller, Ulrich
AU - Baur, Manuela
AU - Roos, Franz F
AU - Brennan, Lorraine
AU - Daniel, Hannelore
AU - Fallaize, Rosalind
AU - Forster, Hannah
AU - Gibney, Eileen R.
AU - Gibney, Mike
AU - Godlewska, Magdalena
AU - Hartwig, Kai
AU - Kolossa, Silvia
AU - Lambrinou, Christina P.
AU - Livingstone, Katherine M.
AU - Lovegrove, Julie A.
AU - Macready, Anna L.
AU - Manios, Yannis
AU - Marsaux, Cyril F M
AU - Martinez, J. Alfredo
AU - Celis-Morales, Carlos
AU - Moschonis, George
AU - Navas-Carretero, Santiago
AU - O'Donovan, Clare B.
AU - San-Cristobal, Rodrigo
AU - Saris, Wim H. M.
AU - Surwiłło, Agnieszka
AU - Traczyk, Iwona
AU - Tsirigoti, Lydia
AU - Walsh, Marianne C.
AU - Woolhead, Clara
AU - Mathers, John C.
AU - Weber, Peter
PY - 2016/1
Y1 - 2016/1
N2 - An efficient and robust method to measure vitamin D (25-hydroxy vitamin D3 (25(OH)D3) and 25-hydroxy vitamin D2 in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3 were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3 levels (P<0·001 for each case). Seasonal effects were modelled by fitting a sine function with a minimum 25(OH)D3 level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson's correlation between the measured values and the predicted values was r 0·65, and the sd of their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.
AB - An efficient and robust method to measure vitamin D (25-hydroxy vitamin D3 (25(OH)D3) and 25-hydroxy vitamin D2 in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3 were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3 levels (P<0·001 for each case). Seasonal effects were modelled by fitting a sine function with a minimum 25(OH)D3 level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson's correlation between the measured values and the predicted values was r 0·65, and the sd of their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.
U2 - 10.1017/S0007114515004298
DO - 10.1017/S0007114515004298
M3 - Article
C2 - 26548417
SN - 0007-1145
VL - 115
SP - 202
EP - 211
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 2
ER -