Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro

Ewelina A Hoffman; Katarzyna Gizelska; Marek Mirowski; Wojciech Mielicki

doi:10.5114/wo.2014.41390

Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro

Ewelina A Hoffman, Katarzyna Gizelska, Marek Mirowski, Wojciech Mielicki

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Abstract

THE AIM OF THE STUDY: To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.MATERIAL AND METHODS: The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.RESULTS: ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose-and time-correlated response within high arsenic concentrations.CONCLUSIONS: Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.

Original language	English
Pages (from-to)	108-12
Number of pages	5
Journal	Contemporary Oncology (Poznan, Poland)
Volume	19
Issue number	2
DOIs	https://doi.org/10.5114/wo.2014.41390
Publication status	Published - 13 May 2015

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10.5114/wo.2014.41390Licence: Other

WO_Art_22439-10 (1)Final published version, 76.9 KBLicence: CC BY

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title = "Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro",

abstract = "THE AIM OF THE STUDY: To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.MATERIAL AND METHODS: The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.RESULTS: ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose-and time-correlated response within high arsenic concentrations.CONCLUSIONS: Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.",

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AU - Hoffman, Ewelina A

AU - Gizelska, Katarzyna

AU - Mirowski, Marek

AU - Mielicki, Wojciech

N1 - © 2015 Termedia Sp. z o. o. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.

PY - 2015/5/13

Y1 - 2015/5/13

N2 - THE AIM OF THE STUDY: To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.MATERIAL AND METHODS: The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.RESULTS: ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose-and time-correlated response within high arsenic concentrations.CONCLUSIONS: Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.

AB - THE AIM OF THE STUDY: To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.MATERIAL AND METHODS: The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.RESULTS: ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose-and time-correlated response within high arsenic concentrations.CONCLUSIONS: Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.

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DO - 10.5114/wo.2014.41390

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SN - 1428-2526

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JO - Contemporary Oncology (Poznan, Poland)

JF - Contemporary Oncology (Poznan, Poland)

IS - 2

ER -

Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro

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