Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics

N. Dimov; J. Gaughran; E. Clancy; T. Barry; T. J. Smith; J. Ducree

doi:10.1109/Transducers.2013.6627325

Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics

N. Dimov, J. Gaughran, E. Clancy, T. Barry, T. J. Smith, J. Ducree

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We present a novel integrated, centrifugo-pneumatic micro-homogenizer (μHomogenizer) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	2548-2551
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6627325
Publication status	Published - 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13

Keywords

bacterial infections
On-disc diagnostics
RNA
spiked blood
three-phase extraction

Access to Document

10.1109/Transducers.2013.6627325

Cite this

Dimov, N., Gaughran, J., Clancy, E., Barry, T., Smith, T. J., & Ducree, J. (2013). Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2548-2551). Article 6627325 https://doi.org/10.1109/Transducers.2013.6627325

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title = "Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics",

abstract = "We present a novel integrated, centrifugo-pneumatic micro-homogenizer (μHomogenizer) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods.",

keywords = "bacterial infections, On-disc diagnostics, RNA, spiked blood, three-phase extraction",

author = "N. Dimov and J. Gaughran and E. Clancy and T. Barry and Smith, \{T. J.\} and J. Ducree",

year = "2013",

doi = "10.1109/Transducers.2013.6627325",

language = "English",

isbn = "9781467359818",

pages = "2548--2551",

booktitle = "2013 Transducers and Eurosensors XXVII",

note = "2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",

}

Dimov, N, Gaughran, J, Clancy, E, Barry, T, Smith, TJ & Ducree, J 2013, Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627325, pp. 2548-2551, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627325

Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics. / Dimov, N.; Gaughran, J.; Clancy, E. et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2548-2551 6627325.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics

AU - Dimov, N.

AU - Gaughran, J.

AU - Clancy, E.

AU - Barry, T.

AU - Smith, T. J.

AU - Ducree, J.

PY - 2013

Y1 - 2013

N2 - We present a novel integrated, centrifugo-pneumatic micro-homogenizer (μHomogenizer) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods.

AB - We present a novel integrated, centrifugo-pneumatic micro-homogenizer (μHomogenizer) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods.

KW - bacterial infections

KW - On-disc diagnostics

KW - RNA

KW - spiked blood

KW - three-phase extraction

UR - https://www.scopus.com/pages/publications/84891690932

U2 - 10.1109/Transducers.2013.6627325

DO - 10.1109/Transducers.2013.6627325

M3 - Conference contribution

AN - SCOPUS:84891690932

SN - 9781467359818

SP - 2548

EP - 2551

BT - 2013 Transducers and Eurosensors XXVII

T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

Dimov N, Gaughran J, Clancy E, Barry T, Smith TJ, Ducree J. Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2548-2551. 6627325 doi: 10.1109/Transducers.2013.6627325

Automated on-disc total RNA extraction from whole blood towards point-of-care for early stage diagnostics

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