Bacillus thuringiensis subsp. aizawai generalized transducing phage φHD248: Restriction site map and potential for fine-structure chromosomal mapping

J. M. Inal; V. Karunakaran; D. R. Jones

Bacillus thuringiensis subsp. aizawai generalized transducing phage φHD248: Restriction site map and potential for fine-structure chromosomal mapping

J. M. Inal, V. Karunakaran, D. R. Jones

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The temperate, generalized transducing phage φHD248 from Bacillus thuringiensis subsp. aizawai (H-serotype 7) was shown previously to mediate transduction within subsp. aizawai and between subsp. aizawai and subsp. kurstaki (H-serotype 3a3b). Here it was found to have a broad host range, plating on 7 out of 14 H-serotypes tested. Cotransduction analysis using φHD248 revealed two linkage groups in H-serotype 7: bio-2-his-2-met-1-cit-1-arg-2-lys-3 and trp-9-purC1-thi-1. Preliminary characterization of the φHD248 genome revealed a linear double-stranded DNA of 47.15 kb. A restriction site map is reported including a total of 10 sites for the enzymes BamHI, BglII, SalII and SstI. Enzyme SalI was found to have a unique cleavage site which if not located in an essential region could be used as a cloning site. The φHD248 genome was also shown to lack cohesive ends and appears to have a partial circular permutation.

Original language	English
Pages (from-to)	1409-1416
Number of pages	8
Journal	Microbiology
Volume	142
Issue number	6
Publication status	Published - Jun 1996

Keywords

Bacillus thuringiensis
Chromosomal mapping
Restriction map
Transducing bacteriophage

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title = "Bacillus thuringiensis subsp. aizawai generalized transducing phage φHD248: Restriction site map and potential for fine-structure chromosomal mapping",

abstract = "The temperate, generalized transducing phage φHD248 from Bacillus thuringiensis subsp. aizawai (H-serotype 7) was shown previously to mediate transduction within subsp. aizawai and between subsp. aizawai and subsp. kurstaki (H-serotype 3a3b). Here it was found to have a broad host range, plating on 7 out of 14 H-serotypes tested. Cotransduction analysis using φHD248 revealed two linkage groups in H-serotype 7: bio-2-his-2-met-1-cit-1-arg-2-lys-3 and trp-9-purC1-thi-1. Preliminary characterization of the φHD248 genome revealed a linear double-stranded DNA of 47.15 kb. A restriction site map is reported including a total of 10 sites for the enzymes BamHI, BglII, SalII and SstI. Enzyme SalI was found to have a unique cleavage site which if not located in an essential region could be used as a cloning site. The φHD248 genome was also shown to lack cohesive ends and appears to have a partial circular permutation.",

keywords = "Bacillus thuringiensis, Chromosomal mapping, Restriction map, Transducing bacteriophage",

author = "Inal, {J. M.} and V. Karunakaran and Jones, {D. R.}",

year = "1996",

month = jun,

language = "English",

volume = "142",

pages = "1409--1416",

journal = "Microbiology",

issn = "1350-0872",

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TY - JOUR

T1 - Bacillus thuringiensis subsp. aizawai generalized transducing phage φHD248

T2 - Restriction site map and potential for fine-structure chromosomal mapping

AU - Inal, J. M.

AU - Karunakaran, V.

AU - Jones, D. R.

PY - 1996/6

Y1 - 1996/6

N2 - The temperate, generalized transducing phage φHD248 from Bacillus thuringiensis subsp. aizawai (H-serotype 7) was shown previously to mediate transduction within subsp. aizawai and between subsp. aizawai and subsp. kurstaki (H-serotype 3a3b). Here it was found to have a broad host range, plating on 7 out of 14 H-serotypes tested. Cotransduction analysis using φHD248 revealed two linkage groups in H-serotype 7: bio-2-his-2-met-1-cit-1-arg-2-lys-3 and trp-9-purC1-thi-1. Preliminary characterization of the φHD248 genome revealed a linear double-stranded DNA of 47.15 kb. A restriction site map is reported including a total of 10 sites for the enzymes BamHI, BglII, SalII and SstI. Enzyme SalI was found to have a unique cleavage site which if not located in an essential region could be used as a cloning site. The φHD248 genome was also shown to lack cohesive ends and appears to have a partial circular permutation.

AB - The temperate, generalized transducing phage φHD248 from Bacillus thuringiensis subsp. aizawai (H-serotype 7) was shown previously to mediate transduction within subsp. aizawai and between subsp. aizawai and subsp. kurstaki (H-serotype 3a3b). Here it was found to have a broad host range, plating on 7 out of 14 H-serotypes tested. Cotransduction analysis using φHD248 revealed two linkage groups in H-serotype 7: bio-2-his-2-met-1-cit-1-arg-2-lys-3 and trp-9-purC1-thi-1. Preliminary characterization of the φHD248 genome revealed a linear double-stranded DNA of 47.15 kb. A restriction site map is reported including a total of 10 sites for the enzymes BamHI, BglII, SalII and SstI. Enzyme SalI was found to have a unique cleavage site which if not located in an essential region could be used as a cloning site. The φHD248 genome was also shown to lack cohesive ends and appears to have a partial circular permutation.

KW - Bacillus thuringiensis

KW - Chromosomal mapping

KW - Restriction map

KW - Transducing bacteriophage

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M3 - Article

AN - SCOPUS:0030004114

SN - 1350-0872

VL - 142

SP - 1409

EP - 1416

JO - Microbiology

JF - Microbiology

IS - 6

ER -

Bacillus thuringiensis subsp. aizawai generalized transducing phage φHD248: Restriction site map and potential for fine-structure chromosomal mapping

Abstract

Keywords

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