Bio-aerosol fluorescence - Detecting and characterising bio-aerosols via UV light-induced fluorescence spectroscopy

Yong-Le Pan, Jay D. Eversole, Paul H. Kaye, Virginia Foot, Ronald G. Pinnick, Steven C. Hill, Michael W. Mayo, Jerold R. Bottiger, Alan Huston, Vasanthi Sivaprakasarn, Richard K. Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


All aspects of fluorescence from a single or a cluster of bio-aerosol particles are treated in this chapter. The following topics are covered: light sources to induce fluorescence, detectors to sense fluorescence, excitation and emission spectra from simulants of known threat bio-aerosols, standards for monitoring aerosols, performance metrics, generation of dry and wet simulant bio-aerosols, examples of commercially available bio-sensors, techniques for enriching aerosol samples by aerodynamic puffing, and the need for an identifier following the enrichment of aerosols sorted according to the aerosols' fluorescence spectra. There is general agreement that fluorescence is a discriminator capable of separating bio- from non-bio-aerosols; however, an appropriate particle identifier has yet to be fully adapted to the monitoring of threat aerosols in the highly variable atmospheric environment.

Original languageEnglish
Title of host publicationOptics of Biological Particles
EditorsA Hoekstra, V Maltsev, G Videen
Place of PublicationDordrecht
PublisherSpringer Nature
Number of pages101
ISBN (Electronic)1-4020-5502-1, 978-1-4020-5502-7
ISBN (Print)1-4020-5501-3, 978-1-4020-5501-0, 1-4020-5500-5, 978-1-4020-5500-3
Publication statusPublished - 2007

Publication series

NameNATO Science Series


  • fluorescence
  • bio-aerosol
  • detection
  • classification


Dive into the research topics of 'Bio-aerosol fluorescence - Detecting and characterising bio-aerosols via UV light-induced fluorescence spectroscopy'. Together they form a unique fingerprint.

Cite this