Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

Niksha Shah; David Naseby

doi:10.1111/jam.12635

Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

Niksha Shah, David Naseby

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

19 Downloads (Pure)

Abstract

Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux

Original language	English
Pages (from-to)	1373-1387
Journal	Journal of Applied Microbiology
Volume	117
Issue number	5
Early online date	3 Oct 2014
DOIs	https://doi.org/10.1111/jam.12635
Publication status	Published - Nov 2014

Access to Document

10.1111/jam.12635

Cite this

@article{27813199074e493f9c99086b535c555d,

title = "Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters",

abstract = "Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux",

author = "Niksha Shah and David Naseby",

year = "2014",

month = nov,

doi = "10.1111/jam.12635",

language = "English",

volume = "117",

pages = "1373--1387",

journal = "Journal of Applied Microbiology",

issn = "1364-5072",

publisher = "Oxford University Press (OUP)",

number = "5",

}

TY - JOUR

T1 - Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

AU - Shah, Niksha

AU - Naseby, David

PY - 2014/11

Y1 - 2014/11

N2 - Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux

AB - Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux

U2 - 10.1111/jam.12635

DO - 10.1111/jam.12635

M3 - Article

SN - 1364-5072

VL - 117

SP - 1373

EP - 1387

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 5

ER -

Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

Abstract

Access to Document

Fingerprint

Cite this