CCR4-associated factor 1 coordinates the expression of Plasmodium falciparum egress and invasion proteins

Bharath Balu; Steven P Maher; Alena Pance; Chitra Chauhan; Anatoli V Naumov; Robert M Andrews; Peter D Ellis; Shahid M Khan; Jing-Wen Lin; Chris J Janse; Julian C Rayner; John H Adams

doi:10.1128/EC.05099-11

CCR4-associated factor 1 coordinates the expression of Plasmodium falciparum egress and invasion proteins

Bharath Balu, Steven P Maher, Alena Pance, Chitra Chauhan, Anatoli V Naumov, Robert M Andrews, Peter D Ellis, Shahid M Khan, Jing-Wen Lin, Chris J Janse, Julian C Rayner, John H Adams

Research output: Contribution to journal › Article › peer-review

Abstract

Coordinated regulation of gene expression is a hallmark of the Plasmodium falciparum asexual blood-stage development cycle. We report that carbon catabolite repressor protein 4 (CCR4)-associated factor 1 (CAF1) is critical in regulating more than 1,000 genes during malaria parasites' intraerythrocytic stages, especially egress and invasion proteins. CAF1 knockout results in mistimed expression, aberrant accumulation and localization of proteins involved in parasite egress, and invasion of new host cells, leading to premature release of predominantly half-finished merozoites, drastically reducing the intraerythrocytic growth rate of the parasite. This study demonstrates that CAF1 of the CCR4-Not complex is a significant gene regulatory mechanism needed for Plasmodium development within the human host.

Original language	English
Pages (from-to)	1257-63
Number of pages	7
Journal	Eukaryotic Cell (EC)
Volume	10
Issue number	9
DOIs	https://doi.org/10.1128/EC.05099-11
Publication status	Published - Sept 2011

Keywords

Animals
Cell Proliferation
Erythrocytes/parasitology
Gene Deletion
Gene Expression
Gene Expression Regulation
Gene Knockout Techniques
Host-Parasite Interactions/genetics
Humans
Life Cycle Stages
Malaria, Falciparum/parasitology
Merozoites/metabolism
Oligonucleotide Array Sequence Analysis/methods
Plasmodium falciparum/genetics
Transcription Factors/genetics

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10.1128/EC.05099-11

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title = "CCR4-associated factor 1 coordinates the expression of Plasmodium falciparum egress and invasion proteins",

abstract = "Coordinated regulation of gene expression is a hallmark of the Plasmodium falciparum asexual blood-stage development cycle. We report that carbon catabolite repressor protein 4 (CCR4)-associated factor 1 (CAF1) is critical in regulating more than 1,000 genes during malaria parasites' intraerythrocytic stages, especially egress and invasion proteins. CAF1 knockout results in mistimed expression, aberrant accumulation and localization of proteins involved in parasite egress, and invasion of new host cells, leading to premature release of predominantly half-finished merozoites, drastically reducing the intraerythrocytic growth rate of the parasite. This study demonstrates that CAF1 of the CCR4-Not complex is a significant gene regulatory mechanism needed for Plasmodium development within the human host.",

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author = "Bharath Balu and Maher, {Steven P} and Alena Pance and Chitra Chauhan and Naumov, {Anatoli V} and Andrews, {Robert M} and Ellis, {Peter D} and Khan, {Shahid M} and Jing-Wen Lin and Janse, {Chris J} and Rayner, {Julian C} and Adams, {John H}",

year = "2011",

month = sep,

doi = "10.1128/EC.05099-11",

language = "English",

volume = "10",

pages = "1257--63",

journal = "Eukaryotic Cell (EC)",

issn = "1535-9778",

publisher = "American Society for Microbiology (ASM)",

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AU - Balu, Bharath

AU - Maher, Steven P

AU - Pance, Alena

AU - Chauhan, Chitra

AU - Naumov, Anatoli V

AU - Andrews, Robert M

AU - Ellis, Peter D

AU - Khan, Shahid M

AU - Lin, Jing-Wen

AU - Janse, Chris J

AU - Rayner, Julian C

AU - Adams, John H

PY - 2011/9

Y1 - 2011/9

N2 - Coordinated regulation of gene expression is a hallmark of the Plasmodium falciparum asexual blood-stage development cycle. We report that carbon catabolite repressor protein 4 (CCR4)-associated factor 1 (CAF1) is critical in regulating more than 1,000 genes during malaria parasites' intraerythrocytic stages, especially egress and invasion proteins. CAF1 knockout results in mistimed expression, aberrant accumulation and localization of proteins involved in parasite egress, and invasion of new host cells, leading to premature release of predominantly half-finished merozoites, drastically reducing the intraerythrocytic growth rate of the parasite. This study demonstrates that CAF1 of the CCR4-Not complex is a significant gene regulatory mechanism needed for Plasmodium development within the human host.

AB - Coordinated regulation of gene expression is a hallmark of the Plasmodium falciparum asexual blood-stage development cycle. We report that carbon catabolite repressor protein 4 (CCR4)-associated factor 1 (CAF1) is critical in regulating more than 1,000 genes during malaria parasites' intraerythrocytic stages, especially egress and invasion proteins. CAF1 knockout results in mistimed expression, aberrant accumulation and localization of proteins involved in parasite egress, and invasion of new host cells, leading to premature release of predominantly half-finished merozoites, drastically reducing the intraerythrocytic growth rate of the parasite. This study demonstrates that CAF1 of the CCR4-Not complex is a significant gene regulatory mechanism needed for Plasmodium development within the human host.

KW - Animals

KW - Cell Proliferation

KW - Erythrocytes/parasitology

KW - Gene Deletion

KW - Gene Expression

KW - Gene Expression Regulation

KW - Gene Knockout Techniques

KW - Host-Parasite Interactions/genetics

KW - Humans

KW - Life Cycle Stages

KW - Malaria, Falciparum/parasitology

KW - Merozoites/metabolism

KW - Oligonucleotide Array Sequence Analysis/methods

KW - Plasmodium falciparum/genetics

KW - Transcription Factors/genetics

U2 - 10.1128/EC.05099-11

DO - 10.1128/EC.05099-11

M3 - Article

C2 - 21803864

SN - 1535-9778

VL - 10

SP - 1257

EP - 1263

JO - Eukaryotic Cell (EC)

JF - Eukaryotic Cell (EC)

IS - 9

ER -

CCR4-associated factor 1 coordinates the expression of Plasmodium falciparum egress and invasion proteins

Abstract

Keywords

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