Centrifugally automated solid-hase purification of RNA

Nikolay Dimov; Jennifer Gaughran; Darren Mc Auley; David Boyle; David J. Kinahan; Jens Ducree

doi:10.1109/MEMSYS.2014.6765625

Centrifugally automated solid-hase purification of RNA

Nikolay Dimov, Jennifer Gaughran, Darren Mc Auley, David Boyle, David J. Kinahan, Jens Ducree

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Citations (Scopus)

Abstract

The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our earlier work the system successfully concatenates event-triggered valving and centrifugally controlled routing in order to collect purified RNA separately from the aqueous and organic wastes. In this article two-stage purification of RNA and sample conditioning through novel high-frequency release and hybrid event-triggered release valves is demonstrated.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	260-263
Number of pages	4
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765625
Publication status	Published - 2014
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States Duration: 26 Jan 2014 → 30 Jan 2014

Conference

Conference	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/Territory	United States
City	San Francisco, CA
Period	26/01/14 → 30/01/14

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10.1109/MEMSYS.2014.6765625

Cite this

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title = "Centrifugally automated solid-hase purification of RNA",

abstract = "The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our earlier work the system successfully concatenates event-triggered valving and centrifugally controlled routing in order to collect purified RNA separately from the aqueous and organic wastes. In this article two-stage purification of RNA and sample conditioning through novel high-frequency release and hybrid event-triggered release valves is demonstrated.",

author = "Nikolay Dimov and Jennifer Gaughran and Auley, {Darren Mc} and David Boyle and Kinahan, {David J.} and Jens Ducree",

year = "2014",

doi = "10.1109/MEMSYS.2014.6765625",

language = "English",

isbn = "9781479935086",

pages = "260--263",

booktitle = "MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

address = "United States",

note = "27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 ; Conference date: 26-01-2014 Through 30-01-2014",

}

Dimov, N, Gaughran, J, Auley, DM, Boyle, D, Kinahan, DJ & Ducree, J 2014, Centrifugally automated solid-hase purification of RNA. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765625, Institute of Electrical and Electronics Engineers (IEEE), pp. 260-263, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 26/01/14. https://doi.org/10.1109/MEMSYS.2014.6765625

Centrifugally automated solid-hase purification of RNA. / Dimov, Nikolay; Gaughran, Jennifer; Auley, Darren Mc et al.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers (IEEE), 2014. p. 260-263 6765625.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Centrifugally automated solid-hase purification of RNA

AU - Dimov, Nikolay

AU - Gaughran, Jennifer

AU - Auley, Darren Mc

AU - Boyle, David

AU - Kinahan, David J.

AU - Ducree, Jens

PY - 2014

Y1 - 2014

N2 - The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our earlier work the system successfully concatenates event-triggered valving and centrifugally controlled routing in order to collect purified RNA separately from the aqueous and organic wastes. In this article two-stage purification of RNA and sample conditioning through novel high-frequency release and hybrid event-triggered release valves is demonstrated.

AB - The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our earlier work the system successfully concatenates event-triggered valving and centrifugally controlled routing in order to collect purified RNA separately from the aqueous and organic wastes. In this article two-stage purification of RNA and sample conditioning through novel high-frequency release and hybrid event-triggered release valves is demonstrated.

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U2 - 10.1109/MEMSYS.2014.6765625

DO - 10.1109/MEMSYS.2014.6765625

M3 - Conference contribution

AN - SCOPUS:84899020065

SN - 9781479935086

SP - 260

EP - 263

BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems

PB - Institute of Electrical and Electronics Engineers (IEEE)

T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014

Y2 - 26 January 2014 through 30 January 2014

ER -

Centrifugally automated solid-hase purification of RNA

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