Centrosomin represses dendrite branching by orienting microtubule nucleation

Cagri Yalgin; Saman Ebrahimi; Caroline Delandre; Li Foong Yoong; Saori Akimoto; Heidi Tran; Reiko Amikura; Rebecca Spokony; Ben Torben-Nielsen; Kevin P. White; Adrian W. Moore

doi:10.1038/nn.4099

Centrosomin represses dendrite branching by orienting microtubule nucleation

Cagri Yalgin, Saman Ebrahimi, Caroline Delandre, Li Foong Yoong, Saori Akimoto, Heidi Tran, Reiko Amikura, Rebecca Spokony, Ben Torben-Nielsen, Kevin P. White, Adrian W. Moore

Centre for Computer Science and Informatics Research

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

33 Downloads (Pure)

Abstract

Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.

Original language	English
Pages (from-to)	1437-45
Number of pages	9
Journal	Nature Neuroscience
Volume	18
Issue number	10
Early online date	31 Aug 2015
DOIs	https://doi.org/10.1038/nn.4099
Publication status	Published - 1 Oct 2015

Access to Document

10.1038/nn.4099

Yalgin_main_v20Accepted author manuscript, 251 KB

Cite this

@article{63d488f4b3564aed82a1bd3cecee1db7,

title = "Centrosomin represses dendrite branching by orienting microtubule nucleation",

abstract = "Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.",

author = "Cagri Yalgin and Saman Ebrahimi and Caroline Delandre and Yoong, {Li Foong} and Saori Akimoto and Heidi Tran and Reiko Amikura and Rebecca Spokony and Ben Torben-Nielsen and White, {Kevin P.} and Moore, {Adrian W.}",

note = "This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nature Neuroscience, after peer review and technical editing by the publisher. To access the final edited work see: https://www.nature.com/articles/nn.4099#rightslink, doi:10.1038/nn.4099.",

year = "2015",

month = oct,

day = "1",

doi = "10.1038/nn.4099",

language = "English",

volume = "18",

pages = "1437--45",

journal = "Nature Neuroscience",

issn = "1097-6256",

publisher = "Nature Research",

number = "10",

}

TY - JOUR

T1 - Centrosomin represses dendrite branching by orienting microtubule nucleation

AU - Yalgin, Cagri

AU - Ebrahimi, Saman

AU - Delandre, Caroline

AU - Yoong, Li Foong

AU - Akimoto, Saori

AU - Tran, Heidi

AU - Amikura, Reiko

AU - Spokony, Rebecca

AU - Torben-Nielsen, Ben

AU - White, Kevin P.

AU - Moore, Adrian W.

N1 - This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nature Neuroscience, after peer review and technical editing by the publisher. To access the final edited work see: https://www.nature.com/articles/nn.4099#rightslink, doi:10.1038/nn.4099.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.

AB - Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.

U2 - 10.1038/nn.4099

DO - 10.1038/nn.4099

M3 - Article

C2 - 26322925

SN - 1097-6256

VL - 18

SP - 1437

EP - 1445

JO - Nature Neuroscience

JF - Nature Neuroscience

IS - 10

ER -

Centrosomin represses dendrite branching by orienting microtubule nucleation

Abstract

Access to Document

Fingerprint

Cite this