A multi-pulse injection strategy for premixed charge compression ignition (PCCI) combustion was investigated in a four-valve, direct-injection diesel engine by a computational fluid dynamics (CFD) simulation using KIVA-3V code coupled with detailed chemistry. The effects of fuel splitting proportion, injection timing, spray angles, and injection velocity were examined. The mixing process and formation of soot and nitrogen oxide (NO) emissions were investigated as the focus of the research. The results show that the fuel splitting proportion and the injection timing impacted the combustion and emissions significantly due to the considerable changes of the mixing process and fuel distribution in the cylinder. While the spray, inclusion angle and injection velocity at the injector exit, can be adjusted to improve mixing, combustion and emissions, appropriate injection timing and fuel splitting proportion must be jointly considered for optimum combustion performance.