Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

Victoria Hutter; Constanze Hilgendorf; Alan Brown; Anne Cooper; Vanessa Zann; David Pritchard; Cynthia Bosquillon

Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

Victoria Hutter, Constanze Hilgendorf, Alan Brown, Anne Cooper, Vanessa Zann, David Pritchard, Cynthia Bosquillon

Research output: Contribution to journal › Meeting abstract › peer-review

Abstract

Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified.

Original language	English
Pages (from-to)	1225-1226
Number of pages	1
Journal	Journal of Pharmacy and Pharmacology
Volume	62
Issue number	10
Publication status	Published - Oct 2010
Event	UK-PHARMSCI Conf - , United Kingdom Duration: 1 Sept 2010 → 3 Sept 2010

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title = "Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model",

abstract = "Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified. ",

author = "Victoria Hutter and Constanze Hilgendorf and Alan Brown and Anne Cooper and Vanessa Zann and David Pritchard and Cynthia Bosquillon",

year = "2010",

month = oct,

language = "English",

volume = "62",

pages = "1225--1226",

journal = "Journal of Pharmacy and Pharmacology",

issn = "0022-3573",

publisher = "Pharmaceutical Press",

number = "10",

note = "UK-PHARMSCI Conf ; Conference date: 01-09-2010 Through 03-09-2010",

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TY - JOUR

T1 - Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

AU - Hutter, Victoria

AU - Hilgendorf, Constanze

AU - Brown, Alan

AU - Cooper, Anne

AU - Zann, Vanessa

AU - Pritchard, David

AU - Bosquillon, Cynthia

PY - 2010/10

Y1 - 2010/10

N2 - Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified.

AB - Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified.

M3 - Meeting abstract

SN - 0022-3573

VL - 62

SP - 1225

EP - 1226

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

IS - 10

T2 - UK-PHARMSCI Conf

Y2 - 1 September 2010 through 3 September 2010

ER -

Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

Abstract

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