TY - JOUR
T1 - Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform
AU - Peticone, Carlotta
AU - Thompson, David De Silva
AU - Dimov, Nikolay
AU - Jevans, Ben
AU - Glass, Nick
AU - Micheletti, Martina
AU - Knowles, Jonathan C.
AU - Kim, Hae Won
AU - Cooper-White, Justin J.
AU - Wall, Ivan B.
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was financially supported by an EPSRC Doctoral Training Grant and Industrial Doctorate Centre in Bioprocess Engineering Leadership (grant number: EP/G034656/1); European Union’s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research Foundation (NRF), Republic of Korea (NRF-2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was also funded in part by the Australian Research Council Discovery Grants Scheme (DP140104217). This work was partly performed at the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia’s researchers.
Funding Information:
We gratefully acknowledge Nicolas Szita, Brian Sullivan for access to the microfluidics laboratory at UCL Department of Biochemical Engineering. The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was financially supported by an EPSRC Doctoral Training Grant and Industrial Doctorate Centre in Bioprocess Engineering Leadership (grant number: EP/G034656/1); European Union?s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research Foundation (NRF), Republic of Korea (NRF-2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was also funded in part by the Australian Research Council Discovery Grants Scheme (DP140104217). This work was partly performed at the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia?s researchers.
Publisher Copyright:
© The Author(s) 2020.
PY - 2020
Y1 - 2020
N2 - Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.
AB - Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.
KW - microfluidics
KW - osteogenic differentiation
KW - phosphate glass
KW - Stem cells
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85093973797&partnerID=8YFLogxK
U2 - 10.1177/2041731420954712
DO - 10.1177/2041731420954712
M3 - Article
AN - SCOPUS:85093973797
VL - 11
JO - Journal of Tissue Engineering (JTE)
JF - Journal of Tissue Engineering (JTE)
ER -